Immobilization of Enzymes and Cells

Immobilization of Enzymes and Cells

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Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation-from the laboratory to industry-of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
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Product details

  • Hardback | 450 pages
  • 154.9 x 236.2 x 30.5mm | 898.13g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2006
  • 217 Illustrations, black and white; XIV, 450 p. 217 illus.
  • 1588292908
  • 9781588292902

Back cover copy

The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.
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Review quote

"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of excellent reference work that will have long lasting value." -Quarterly Review of Biology

" of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

" ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst
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Table of contents

Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?
Jose M. Guisan

Immobilization of Enzymes: A Literature Survey
Beatriz M. Brena and Franciso Batista-Viera

Cross-Linked Enzyme Aggregates
Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen

Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups
Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente

Glutaraldehyde in Protein Immobilization: A Versatile Reagent
Lorena Betancor, Fernando Lopez-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan

Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques
Manfred T. Reetz

Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques
Monica Campas and Jean-Louis Marty

Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers
Ipsita Roy and Munishwar N. Gupta

Affinity Immobilization of Tagged Enzymes
Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty

Bioaffinity Immobilization
Ipsita Roy and Munishwar N. Gupta

One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports
Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando Lopez-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran
Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan

Purification, Immobilization,Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports
Jose M. Palomo, Gloria Fernandez-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan

Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads (R)
Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando Lopez-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernandez-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente

Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports
Tamara Montes, Fernando Lopez-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach
Alankar Vaidya and Lutz Fischer

Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups
Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta

Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers
Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando Lopez-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente

Immobilization of Enzymes on Magnetic Particles
Martina Koneracka, Peter Kopcansk'y , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira

Immobilization of Proteins on Gold Surfaces
Jose M. Abad, Marcos Pita, and Victor M. Fernandez

Immobilization of Enzymes on Electrodes
Gilvanda Silva Nunes and Jean-Louis Marty

Immobilization of Enzymes for Use in Organic Media
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