Human Papillomaviruses

Human Papillomaviruses : Methods and Protocols

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Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Product details

  • Hardback | 496 pages
  • 158 x 232 x 28mm | 938.95g
  • Totowa, NJ, United States
  • English
  • 2006 ed.
  • 153 Illustrations, black and white; XVI, 496 p. 153 illus.
  • 1588293734
  • 9781588293732

Back cover copy

Human papillomaviruses (HPV) are an important class of pathogens responsible for a variety of diseases, including cervical cancer, the second most commonly found female cancer worldwide. In Human Papillomaviruses: Methods and Protocols, leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of this formidable virus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Human Papillomaviruses: Methods and Protocols offers both novice and experienced investigators a set of highly successful analytical tools for unlocking the secrets of the human papillomaviruses and their pathologies.
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Table of contents

Identification of New Papillomavirus Types
Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst

Identification of HPV Variants
John Cason, Jon Bible, and Christine Mant

Histochemical Analysis of Cutaneous HPV-Associated Lesions
Kiyofumi Egawa

Histological Analysis of Cervical Intraepithelial Neoplasia
Michael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, and David Jenkins

Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
Woei Ling Peh and John Doorbar

Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Rashmi Seth, John Rippin, Li Guo, and David Jenkins

Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers
Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz

Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
Jan P. A. Baak and Arnold-Jan Kruse

HPV DNA Detection and Typing in Cervical Scrapes
Peter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, Rene P. Pol, and Chris J. L. M. Meijer

HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
Maurits de Koning, Linda Struijk, Mariet Feltkamp, and Jan ter Schegget

Establishing HPV-Containing Keratinocyte Cell Lines From Tissue Biopsies
Margaret Anne Stanley

Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic 'Raft' Cultures
Paul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, and Tomomi Nakahara

Differentiation of HPV-Containing Cells Using Organotypic 'Raft' Culture or Methylcellulose
Regina Wilson and Laimonis A. Laimins

Propagation of Infectious, High-Risk HPV in Organotypic 'Raft' Culture
Margaret E. McLaughlin-Drubin and Craig Meyers

Retrovirus-Mediated GeneTransfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic 'Raft' Cultures
N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker

The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
William Bonnez

The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
Janet L. Brandsma

Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
Yong Liu, Hong You, and Paul L. Hermonat

Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Peter C. Angeletti

Analysis of the Regulation of Viral Transcription
Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard

Analysis of HPV Transcription by RPA
Jason M. Bodily and Craig Meyers

Analysis of Regulatory Motifs Within HPV Transcripts
Sarah A. Cumming and Sheila V. Graham

Detection of HPV Transcripts by Nested RT-PCR
Christine Mant, Barbara Kell, and John Cason

Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
Biing Yuan Lin, Thomas R. Broker, and Louise T. Chow

Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR
Iain M. Morgan and Ewan R. Taylor

Analysis of E7/Rb Associations
Sandra Caldeira, Wen Dong, and Massimo Tommasino

Transformation Assays for HPV Oncoproteins
Paola Massimi and Lawrence Banks

Analysis of Adeno-Associated Virus and HPV Interaction
Paul L. Hermonat, Hong You, C. Maurizio Chiriva-Internati, and Yong Liu

In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
Miranda Thomas and Lawrence Banks

Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
Anna M. Kowalczyk, Geraldine E. Roeder, Katie Green, David J. Stephens, and Kevin Gaston

Codon Optimization of Papillomavirus G
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