Hormone Assays in Biological Fluids

Hormone Assays in Biological Fluids

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Description

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.
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Product details

  • Hardback | 258 pages
  • 154 x 236 x 20mm | 521.64g
  • Totowa, NJ, United States
  • English
  • 2006 ed.
  • IX, 258 p.
  • 1588290050
  • 9781588290052

Back cover copy

Although automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.
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Table of contents

Immunoassay Techniques
Michael J. Wheeler

High-Performance Liquid Chromatography for Hormone Assay
John W. Honour

Gas Chromatography-Mass Spectrometry
John W. Honour

An Introduction to PCR Techniques
Gill Rumsby

Methods for the Investigation of Thyroid Function
Christine R. Squire

Assays for LH, FSH, and Prolactin
Michael J. Wheeler

Adrenocorticotropic Hormone and Related Peptides
Les A. Perry

Measurement of Glucocorticoids in Biological Fluids
Geoff Holder

Urinary Steroid Profiling
Norman F. Taylor

Measurement of Aldosterone in Blood
Marion L. Cawood

Measurement of Plasma Renin Activity
Marion L. Cawood

Measurement of Androgens
Michael J. Wheeler

Measurement of Gut Hormones in Plasma
Shahrad Taheri, Mohammad Ghatei, and Steve Bloom

Measurement of Melatonin and 6-Sulphatoxymelatonin
Benita Middleton

Index
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Review Text

From the reviews:

"Interest in hormone diagnostics has developed dramatically over the last decades ... . In summary, this book is written by knowledgeable experts in the field of hormone analysis in biological fluids ... . It provides a valuable collection of assays, described in a practical way, so they can be rapidly implemented by laboratorians looking for hormone assays suitable for research purposes or as alternatives to commercial immunoassays." (Linda M. Thienpont, Clinical Chemistry, Vol. 52 (9), 2006)

"...a wonderful resource for all thoe who, at some point, face decisions about the most suitable hormone assay for a particular situation." -TRENDS in Endocrinology and Metabolism
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Review quote

From the reviews:





"Interest in hormone diagnostics has developed dramatically over the last decades ... . In summary, this book is written by knowledgeable experts in the field of hormone analysis in biological fluids ... . It provides a valuable collection of assays, described in a practical way, so they can be rapidly implemented by laboratorians looking for hormone assays suitable for research purposes or as alternatives to commercial immunoassays." (Linda M. Thienpont, Clinical Chemistry, Vol. 52 (9), 2006)





"...a wonderful resource for all thoe who, at some point, face decisions about the most suitable hormone assay for a particular situation." -TRENDS in Endocrinology and Metabolism
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