High Performance Liquid Chromatography and Capillary Electrophoresis : Principles and Practices
HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes.
- Hardback | 280 pages
- 156.5 x 233.7 x 19.3mm | 598.75g
- 21 Jul 1997
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
- b&w illustrations, references, bibliography
Table of contents
High Performance Liquid Chromatography, Introduction. Classification of Liquid Chromatographic Methods. Basic Concepts. Summary of Major Concepts. References. Separations in High Performance Liquid Chromatography, Introduction. Normal Phase Chromatography. Reversed Phase Chromatography. Hydrophobic Interaction. Ion Exchange Chromatography. Size Exclusion Chromatography. Affinity Chromatography. Chiral Separations. Summary of Major Concepts. References. Instrumentation for High Performance Liquid Chromatography, Introduction. Solvent Delivery Systems. Solvent Degassing. Isocratic Versus Gradient Pumping Systems. Sample Introduction. Columns. Detectors. Sample Preparation. Troubleshooting. Summary of Major Concepts. References. Capillary Electrophoresis, Introduction. Classification of Electrophoretic Modes. Basic Concepts. Summary of Major Concepts. Separations in Capillary Electrophoresis, Introduction. Capillary Zone Electrophoresis. Micellar Electrokinetic Capillary Chromatography. Capillary Gel Electrophoresis. Capillary Electromatography. Chiral Capillary Electrophoresis. Capillary Isoelectric Focusing. Capillary Isotachophoresis. Summary of Major Concepts. References. Instrumentation for Capillary Electrophoresis, Introduction. High Voltage Power Supply. Sample Injection. Capillaries. Detection. Fraction Collection. Sample Preparation. Troubleshooting. Summary of Major Concepts. References. Data Manipulation, Introduction. Identification of Peaks. Quantification. Summary of Major Concepts. References. Miniaturization, Introduction. Classification. Applications of Miniaturized LC. Separations on a Chip. Summary of Major Concepts. References.