Hepatocyte Transplantation: Preliminary Entry 2077
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Hepatocyte Transplantation: Preliminary Entry 2077 : Methods and Protocols

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Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.show more

Product details

  • Hardback | 231 pages
  • 190.5 x 261.62 x 17.78mm | 703.06g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2009 ed.
  • 9 black & white tables, biography
  • 1588298833
  • 9781588298836

Back cover copy

Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease. "show more

Table of contents

1. Human Hepatocyte Transplantation Overview Juliana Puppi and Anil Dhawan 2. Isolation of Human Hepatocytes Ragai R. Mitry 3. An Optimised Method for Cryopreservation of Human Hepatocytes Claire Terry and Robin D. Hughes 4. Liver Cell Culture Techniques Jose V. Castell and Maria Jose Gomez-Lechon 5. In vitro Assays for Induction of Drug Metabolism Brian G. Lake, Roger J. Price, Amanda M. Giddings and David G. Walters 6. Hepatocyte Apoptosis Mustapha Najimi, Francoise Smets and Etienne Sokal 7. Small Animal Models of Hepatocyte Transplantation Jurgen Seppen, Ebtisam El Filali and Ronald Oude Elferink 8. Hepatocyte Transplantation Techniques: Large Animal Models Anne Weber, Marie-Therese Groyer-Picard and Ibrahim Dagher 9. Cell Transplant Techniques: Engraftment Detection of Cells Robert A. Fisher and Valeria R. Mas 10. Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation Yao-Ming Wu and Sanjeev Gupta 11. Ex vivo Gene Transfer into Hepatocytes Xia Wang, Prashant Mani, Debi P. Sarkar, Namita Roy-Chowdhury and Jayanta Roy-Chowdhury 12. Sources of Adult Hepatic Stem Cells: Haematopoietic Rosemary Jeffery, Richard Poulsom and Malcolm R. Alison 13. Production of Hepatocyte-like Cells from Human Amnion Toshio Miki, Fabio Marongiu, Ewa C.S. Ellis, Ken Dorko, Keitaro Mitamura, Aarati Ranade, Roberto Gramignoli and Stephen C. Strom 14. Generation of Hepatocytes from Human Embryonic Stem Cells Niloufar Safinia and Stephen L. Minger 15. Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells Yue Wu, Chetan C. Shatapathy and Stephen L. Minger 16. Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro Ruth Joplin and Stivelia Kachilele 17. Liver Cell Labelling with MRI Contrast Agents Michel Modo, Thomas J. Meade and Ragai R. Mitry 18. Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory Sharon C. Lehecshow more