Hepatitis C

Hepatitis C : Methods and Protocols

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Description

When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the ?rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus ?eld was something I might want to work on in the future. At the time, the ?rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the ?eld at that time.
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Product details

  • Hardback | 466 pages
  • 192 x 262 x 28mm | 1,102.22g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2009
  • 23 Tables, black and white; 1 Illustrations, color; 73 Illustrations, black and white; XXII, 466 p. 74 illus., 1 illus. in color.
  • 1588299708
  • 9781588299703

Review quote

From the reviews of the second edition:

"This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. ... The chapters are authored by acknowledged experts and are authoritatively written. ... Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community ... this book will be a success and should adorn the shelves of many laboratories." (David J. Rowlands, Microbiology Today, July, 2009)
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Back cover copy

With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.
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Table of contents

PART I: DETECTION AND QUANTIFICATION OF HCV

1. Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays
Helene Le Guillou-Guillemette and Francoise Lunel-Fabiani

2. Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen
Filippo Ansaldi and Giancarlo Icardi

3. Immunohistochemical Detection of HCV Proteins in Liver Tissue
Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, and Isabelle Chemin

PART II: HCV NONMENCLATURE, GENOTYPING, AND QUASISPECIES

4. Nomenclature and Numbering of the Hepatitis C Virus
Carla Kuiken and Peter Simmonds

5. A Real-Time Taqman (R) Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates
Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J.M. Alexander, and Martin D. Curran

6. Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR1) by In Vitro Translation and Mass Spectrometry
Carmen Yea, Melissa Ayers, and Raymond Tellier

PART III: HCV REPLICATION IN VITRO

7. Purification and Crystallization of NS5A Domain I of Hepatitis C Virus
Joseph Marcotrigiano and Timothy L. Tellinghuisen

8. NS5A Phosphorylation and Hyperphosphorylation
Petra Neddermann

9. Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies
Stefania Di Marco, Edwin Rydberg, Matthew Bottomley, and Andrea Carfi

10. Structural and Functional Analysis of the HCV p7 Protein
Nathalie Saint, Roland Montserret, Christophe Chipot, and Francois Penin

11. HCV Replicons: Overview and Basic Protocols
Volker Lohmann

12. Reverse Transcription PCR Based Sequence Analysis of Hepatitis C Virus RepliconRNA
Timothy L. Tellinghuisen and Brett D. Lindenbach

13. Studying HCV RNA Synthesis In Vitro with Replication Complexes
Wengang Yang and Mingjun Huang

14. Proteomics Study of the Hepatitis C Virus Replication Complexes
Kyungsoo Chang, Tianyi Wang, and Guangxiang Luo

15. Investigation of the Hepatitis C Virus Replication Complex
Volker Brass, Rainer Gosert, and Darius Moradpour

16. Regulation of Interferon Regulatory Factor-3-dependent Innate Immunity by the HCV NS3/4A Protease
Kui Li

17. Selection and Characterization of Drug Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
Jason M. Robotham, Heather B. Nelson, and Hengli Tang

18. High Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System
Weidong Hao and Rohit Duggal

19. Inhibition of HCV Replication by Small Interfering RNA
Ratna B. Ray and Tastuo Kanda

PART IV: HCV ENTRY AND INFECTION

20. Cellular Receptors and HCV Entry
Mike Flint and Donna M. Tscherne

21. Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)
Birke Bartosch and Francois-Loic Cosset

22. Screening of Small-molecule Compounds as Inhibitors of HCV Entry
Jian-Ping Yang, Demin Zhou and Flossie Wong-Staal

23. Isolation of the JFH-1 Strain and Development of an HCV Infection System
Takaji Wakita

24. Measuring HCV Infectivity Produced in Cell Culture and In Vivo
Brett D. Lindenbach

25. Genotype 1a HCV (H77S) Infection System
MinKyung Yi and Stanley M. Lemon

26. Full Length Infectious HCV Chimeras
Thomas Pietschmann

27. Adaptation of the Hepatitis C Virus to Cell Culture
Artur Kaul, Ilka Woerz and Ralf
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Review Text

From the reviews of the second edition: "This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. ... The chapters are authored by acknowledged experts and are authoritatively written. ... Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community ... this book will be a success and should adorn the shelves of many laboratories." (David J. Rowlands, Microbiology Today, July, 2009)
show more