Hepatitis B and D Protocols: Detection, Genotypes, and Characterization v. 1

Hepatitis B and D Protocols: Detection, Genotypes, and Characterization v. 1

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Despite the availability of an effective vaccine, there are still 400 million people, worldwide who are chronically infected with hepatitis B virus (HBV). For them, the vaccine, as currently applied, has no value. Given the possible consequences of HBV infection, the number of those chronically infected with HBV presents an enormous public health challenge. For example, the major etiology of hepatocellular carcinoma (HCC) is chronic infection with HBV. Although fifth in cancer incidence, worldwide, HCC/liver cancer is the third leading cause of cancer death. The high mortality as- ciated with HCC arises because the disease is often detected late and is unresponsive to treatment. The number of deaths caused by PHCC is expected to rise over the next 20 years. Those chronically infected with HBV have a life risk of death to HCC of between 10 and 25%. Even the limited efficacy of drugs for the treatment of chronic HBV helps underscore the point that this disease is responsive to therapy. Drugs that target the polymerase (e. g. , hepsera and lamivudine) and interferon alpha represent two distinct strategies and show that both conventional antiviral and immunothe- peutic approaches can be used in management. However, the current inventory of therapeutics is inadequate. Interferon alpha is of limited value, only parenterally ava- able, and fraught with adverse reactions.show more

Product details

  • Hardback | 376 pages
  • 154.9 x 233.7 x 25.4mm | 680.4g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2004 ed.
  • 109 black & white illustrations, 1 colour illustrations, biography
  • 1588291057
  • 9781588291059

Review quote

From the reviews: "Both volumes provide an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D. A major attribute of the volumes is that as they cover a wide range of subjects, the reader has the opportunity to access information they would not normally encounter. Volumes 1 and 2 are an excellent reference source and the methodologies described present the opportunity for both new and experienced researchers to study the molecular aspects of HBV and HDV infection."-SGM Quarterly "...an excellent reference for background information and detailed experimental investigations for both Hepatitis B and Hepatitis D..." - Microbiology Today "The book is an extremely comprehensive collection of valuable modern protocols on detection of HBV DNA in serum and liver ... . In summary, the general layout of the book is of excellent quality. Details are painstakingly provided so that sophisticated readers can profit from first hand top quality information derived from people who have personally worked at the bench. The substantial number of references will also allow easy access to the specific literature in the field." (M. Mondelli, Digestive and Liver Disease, Vol. 37, 2005)show more

Back cover copy

Despite the availability of an effective vaccine, there are still 400 million people worldwide who are chronically infected with the hepatitis B and D viruses (HBV and HDV), and more work still needs to be done in all of the key areas of managing the disease. In Hepatitis B and D Protocols, leading investigators and clinicians have joined forces to create a broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Taken together, Hepatitis B and D Protocols, Volume 1: Detection, Genotypes, and Characterization and Volume 2: Immunology, Model Systems, and Clinical Studies offer both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, as well as an essential resource for finding new therapies to treat chronically infected patients.show more

Table of contents

Part I. Detection of Viral Markers Overview of Commercial HBV Assay Systems Stefan Zeuzem Detection of HBV DNA in Serum Using a PCR-Based Assay Hau Tim Chung Detection of HBV DNA by Oligonucleotide Probing Hsiang Ju Lin Detection of HBV RNA in Serum of Patients Wei Zhang, Hans Jorg Hacker, Maria Mildenberger, Qin Su, and Claus H. Schroder Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR Scott Bowden, Kathy Jackson, Margaret Littlejohn, and Stephen Locarnini In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections Vicky C. H. Lai and Johnson Y. N. Lau Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis Wolfram H. Gerlich, Ulrike Wend, and Dieter Glebe In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approach Jane W. S. Fang and Johnson Y. N. Lau Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting Jie Liu and Mark A. Feitelson Detection of Serum HDV-RNA by RT-PCR Antonina Smedile, Maria Grazia Niro, and Mario Rizzetto Nonradioisotopic In Situ Hybridization for HDV RNA Francesco Negro Detection and Characterization of Small and Large HDV Antigens Thomas B. Macnaughton and Michael M. C. Lai Immunohistochemical Detection of Hepatitis Delta Antigen Karim Abid and Francesco Negro Part II. Viral Genotypes and Variants HBV Vaccine-Escape Variants Ashraf A. Basuni and William F. Carman HBV Genotyping and Analysis for Unique Mutations Anna Ayres, Stephan Locarnini, and Angeline Bartholomeusz A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis BVirus Chiaho Shih and Ta-Tung Thomas Yuan Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion Chiaho Shih and Pei-Ching Tai Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay Sija De Gendt, Fred Shapiro, Jelena Juras, Els Van Assche, Geert Maertens, and Erwin Sablon Part III. Molecular Biological Characterization Processing of Hepatitis B Virus Surface Proteins Volker Bruss Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus Stephan Urban Nucleoprotein Transport of HBV Capsid Particles Michael Kann Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian cells Jie Li, Yanyan Zheng, Jinah Choi, and Jing-hsiung Ou Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions Fabien Zoulim, ShuPing Tong, and Christian Trepo A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein Chiaho Shih and Ta-Tung Thomas Yuan Studying DHBV Polymerase by In Vitro Transcription and Translation Jianming Hu Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System Lisa Lott, Lena Notvall, and Robert E. Lanford Localization of Duck Hepatitis B Virus Polymerase Within Cells Ermei Yao and John E. Tavis Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice Zhenming Xu and Jing-hsiung Ou Transcriptional Control of Hepatitis B Virus Anneke K. Raney In Vitro Reconstitution of e-Dependent Duck Hepatitis B Virus Replication Initiation Jurgen Beck and Michael Nassal Hepatitis Bshow more