Hematopoietic Stem Cell Protocols: Preliminary Entry 2239

Hematopoietic Stem Cell Protocols: Preliminary Entry 2239

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This revised edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. The book provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations, and offers a variety of transplantation approaches to measure HSC function in vivo. This is a much needed technical resource in the critically important field of stem cell investigation.show more

Product details

  • Hardback | 312 pages
  • 157.48 x 236.22 x 25.4mm | 657.71g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2008
  • 12 black & white tables, biography
  • 158829868X
  • 9781588298683
  • 2,415,040

Back cover copy

With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation. "show more

Table of contents

Part I. Overview 1. The Expanding Tool Kit for Hematopoietic Stem Cell Research William Tse and Kevin D. Bunting Part II. Stem Cell Enrichment and Analysis 2. Isolation of Quiescent Murine Hematopoietic Stem cells by Homing Properties Tarja A. Juopperi and Saul J. Sharkis 3. Mobilization of Hematopoietic Stem and Progenitor cells (HSPC) in Mice Simon N. Robinson and Ronald P. van Os 4. Transmigration of Human CD34+ cells Seiji Fukuda and Louis M. Pelus 5. Flow Cytometry Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells Hongmei Shen, Matthew Boyer, and Tao Cheng 6. Analysis of Apoptosis in HSC by Flow Cytometry William G. Kerr Part III. In vitro Assays and Differentiation 7. In vitro Hematopoietic Differentiation of Murine Embryonic Stem (ES) Cells Jinhua Shen and Cheng-Kui Qu 8. Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman 9. In vitro Human T Cell Development Directed by Notch-ligand Interactions Geneve Awong, Ross N. La Motte-Mohs and Juan Carlos Zuniga-Pflucker 10. In vitro Assays for Cobblestone Area Forming Cells, LTC-IC, and CFU-C Ronald van Os, Bertien Dethmers-Ausema, and Gerald de Haan Part IV. Transplantation Assays for Mouse and Human HSC 11. HSC Transplant in Mice by Intra Femoral Injection Yi Zhao and Yuxia Zhan 12. Hematopoietic Stem Cell Transplant into Non-myeloablated W/Wv mice to Detect Steady-state Engraftment Defects Zhengqi Wang and Kevin D. Bunting 13. Isolation and FunctionalCharacterization of Side Population Stem Cells Jonathan B. Johnnidis and Fernando D. Camargo 14. Transplantation of Chimeric Fetal Liver to Study Hematopoiesis Sigrid Eckardt and K. John McLaughlin 15. Immunodeficient Mouse Models to Study Human Stem Cell-mediated Tissue Repair Ping Zhou, Sarah Hohm, Ben Cappocia, Louisa Wirthlin, David Hess, Dan Link, and Jan Nolta Part V. Genetic Modification of HSCs and Imaging Engraftment 16. Retroviral Transduction of Murine Hematopoietic Stem Cells Peter Haviernik, Yi Zhang, and Kevin D. Bunting 17. Lentiviral Gene Transduction of Mouse and Human Stem Cells Zhaohui Ye, Xiaobing Yu, and Linzhao Cheng 18. Retroviral Integration Site Analysis in Hematopoietic Stem Cells Olga S. Kustikova, Christopher Baum, and Boris Fehse 19. A Detailed Protocol for BAC Recombineering to Study Essential Genes in Stem Cells Andriy Tsyrulnyk and Richard Moriggl 20. Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models Yuan Lin, Joe Molter, Zhenghong Lee, Stanton L. Gersonshow more

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