Hedgehog Signaling Protocols

Hedgehog Signaling Protocols

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Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.
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Product details

  • Hardback | 256 pages
  • 152.4 x 231.14 x 17.78mm | 635.03g
  • Totowa, NJ, United States
  • English
  • 2007 ed.
  • 18 Tables, black and white; 7 Illustrations, color; 38 Illustrations, black and white; XVII, 256 p. 45 illus., 7 illus. in color.
  • 158829692X
  • 9781588296924

Back cover copy

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.
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Table of contents

Chapter 1. Purifying the Hedgehog protein and its variants by Darren P.
Baker, Frederick R. Taylor and R. Blake Pepinsky
Chapter 2. Application of Sonic hedgehog to the developing chick limb
by Eva Tiecke and Cheryll Tickle
Chapter 3. Manipulation of Hedgehog signaling in Xenopus by means of
embryo microinjection and application of chemical inhibitors by Thomas
Hollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas Pieler
Chapter 4. Isolation of rat telencephalic neural explants to assay Shh
GABAergic interneuron differentiation-inducing activity by Rina Mady
and Jhumku D. Kohtz
Chapter 5. Genetic Analysis of the Vertebrate Hedgehog Signaling
Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo by
Sudipto Roy
Chapter 6. Efficient Manipulation of Hedgehog/GLI Signaling using
Retroviral Expression Systems by Maria Kasper, Gerhard Regl, Thomas
Eichberger, Anna-Maria Frischauf and Fritz Aberger
Chapter 7. Cell Surface Marker and Cell Cycle Analysis, Hedgehog
Signaling, and Flow Cytometry by Kristina Detmer and Ronald E. Garner
Chapter 8. Detecting tagged Hedgehog with Intracellular and
Extracellular Immunocytochemistry for functional analysis by Ainhoa
Callejo, Luis Quijada and Isabel Guerrero
Chapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled
with Fluorescent in situ Hybridization of Hedgehog Target Genes by
Armel Gallet and Pascal P. Therond
Chapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vector
for temporally and spatially controlled RNAi in Drosophila by Eric
Marois and Suzanne Eaton
Chapter 11. Germline clone analysis for maternally acting Drosophila
Hedgehog components by Erica M. Selva and Beth E. Stronach
Chapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatic
tissues by Christine M. Bankers and Joan E. Hooper
Chapter 13. GAL4/UAS targeted gene expression for studyingDrosophila
Hedgehog signaling by Denise Busson and Anne-Marie Pret
Chapter 14. Biochemical Fractionation of Drosophila Cells by Melanie
Stegman and David Robbins
Chapter 15. Using Immunoprecipitation to Study Protein-protein
Interactions in the Hedgehog Signaling Pathway by Chao Tong and Jin
Chapter 16. Sequence analyses to study the evolutionary history and
cis-regulatory elements of Hedgehog genes by Ferenc Muller and
Anne-Gaelle Borycki
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