Guide to Techniques in Glycobiology: Volume 230

Guide to Techniques in Glycobiology: Volume 230

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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Product details

  • Hardback | 567 pages
  • 152 x 229 x 25.4mm | 950g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0121821315
  • 9780121821319

About John N. Abelson

WILLIAM J. LENNARZ received his B.S. in Chemistry from Pennsylvania State University and a Ph.D. in Organic Chemistry from the University of Illinois. Subsequently he carried out postdoctoral work at Harvard with Konrad Bloch on fatty acid biosynthesis. In 1962 he was appointed Assistant Professor at Johns Hopkins in the Department of Physiological Chemistry. After promotion to Associate Professor in 1967, and full Professor in 1971, he remained at Hopkins until 1983. At that time, he was appointed Robert A. Welch Professor and Chair of the Department of Biochemistry and Molecular Biology at the University of Texas Cancer Center, M.D. Anderson Hospital. In 1989 he became a Leading Professor and Chair of the Department of Biochemistry and Cell Biology at SUNY at Stony Brook. In 1990 he founded and became Director of the Institute for Cell and Developmental Biology at Stony Brook. Dr. Lennarz has served on many national and international committees. He has served as President of the Biochemistry Chairman's Organization, President of the American Society for Biochemistry and Molecular Biology and President of the Society for Glycobiology. He was a member of the Executive Committee of the International Union of Biochemistry and Molecular Biology for almost a decade. He has presented special lectures at the University of Notre Dame, the NIH, the University of West Virginia, Johns Hopkins University, Florida State University, the University of California at San Diego, the University of Arkansas, Indiana University and the Medical College of Virginia. He is a member of the National Academy of Sciences. The focus of his early work was on lipids and bacterial cell surfaces. More recent efforts have been in the structure, biosynthesis and function of cell surface glycoproteins. The biosynthesis studies initially were carried out in liver and oviduct, but these efforts now are focused in yeast. The functional studies have concentrated on the role of cell surface glycoproteins in fertilization and early development in the sea urchin and, more recently, the frog. He served as Distinguished Professor and Chair of his department. He now is Distinguished Professor Emeritus.
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Review quote

Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
"Incomparably useful."
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."
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Table of contents

Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry, R.K. Merkle and I. Poppe; metabolic radiolabeling of glycoconjugates, A. Varki; nonmetabolic radiolabeling and tagging of glyconconjugates, C.G. Gahmberg and M. Tolvanen; enzymatic deglycosylation of asparagine-linked glycans - purification, properties, and specificity of oligosaccharide-cleaving enzymes from flavobacterium meningosepticum, A.L. Tarentino and T.H. Plummer; release of oligosaccharides from glycoproteins by hydrazinolysis, T.P. Patel and R.B. Parekh; use of lectins in analysis of glycoconjugates, R.C. Cummings; saccharide linkage analysis using methylation and other techniques, R. Geyer and H. Geyer; mass spectrometry of carbohydrate-containing bipolymers, A.Dell et al; (1)H nuclear magnetic resonance spectroscopy of carbohydrate chains of glycoproteins, H. van Halbeek; determination of stalic acids, G. Reuter and R. Schauer; size fractionation of oligosaccharides, A. Kobata; high-pH anion-exchange chromatography of glycoprotein-derived carbohydrates, M.R. Hardy and R.R. Townsend; high-performance liquid chromatography of pyridylaminated saccharides, S. Hase; high-performance liquid chromatography of oligosaccharides, J.E. Baenziger; high-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides, P. Jackson; capillary electrophoresis of oligosaccharides, R.J. Linhardt; glycosidases in structural analysis, G.S. Jacob and P. Scudder; glycosyltransferases in glycobiology, M.M. Palcic; glycosidase inhibitors in study of glycoconjugates, G.P. Kaushal and A.D. Elbein; synthesis and use of azido-substituted nucleoside diphosphate sugar photoaffinity analogs, A. Radominska and R.R. Drake; glycoform analysis of glycoproteins, R.B. Parekh; isolation of glycosphingolipids, R.L. Schnaar; thin-layer chromatography of glycosphingolipids, R.L. Schnaar and L.K. Needham; isolation and characterization of proteoglycans, V.C. Hascall et al; structural analysis of glycosylphosphatidylinositol anchors, A.K. Menon; detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins, E.P. Roquemore et al; identification of polysialic acids in glycoconjugates, J. Ye et al; neoglycolipds - probes of oligosaccharide structure, antigenicity, and function. T. Feizi et al.
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