Guide to Protein Purification

Guide to Protein Purification

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"The Guide to Protein Purification" is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher. It contains procedures newly written for this volume; and provides extensive practical information. It clearly describes rationale and strategies for protein and enzyme purification. It offers personal perspectives on enzyme purification by eminent researchers.
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Product details

  • Paperback | 894 pages
  • 190.8 x 246.1 x 43.4mm | 1,423.26g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0122135857
  • 9780122135859

Review quote

"In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years." TIB-TECH
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Table of contents

Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Personal Perspectives on Enzyme Purification. Index.
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