Green Fluorescent Protein: Volume 302
This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). The OVID database (including MEDLINE, Current Contents, and other sources) lists nine references to GFP for the ten-year period 1985-1994. In contrast, in less than four years thereafter, over 500 references are listed, a testament to the rapid growth of interest in this probe.
This volume documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies-such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. The volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
- Hardback | 490 pages
- 160.5 x 236.2 x 27.7mm | 940.55g
- 13 Apr 1999
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Other books in this series
11 Oct 2012
19 Oct 2012
13 Jul 2017
01 Dec 2009
15 Nov 2013
27 Mar 2012
21 Aug 2013
09 Aug 2017
18 Aug 2017
19 Aug 2013
12 Sep 2013
15 Jun 2015
27 Mar 2012
27 Jan 2017
26 Jan 2016
04 May 2010
14 Mar 2011
Table of contents
S. Kupzig, S. San Lee and G. Banting, Membrane Trafficking.
C. Kaether and H.-H. Gerdes, Monitoring of Protein Secretion with Green Fluorescent Protein.
R.M. Hoffman, Green Fluorescent Protein to Visualize Cancer Progression and Metastasis.
X. Zhao, T. Duong, C.-C. Huang, S.R. Kain and X. Li, Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters.
S.R. Kain and J.-T. Ma, Early Detection of Apoptosis with Annexin V-Enhanced Green Fluorescent Protein.
M. Maniak, Green Fluorescent Protein in the Visualization of Particle Uptake and Fluid-Phase Endocytosis.
F. Hanakam and G. Gerisch, Monitoring Intracellular Shuttling of Histidine-rich pH-Sensor Proteins Tagged with Green Fluorescent Protein.
S.R. Cronin and R.Y. Hampton, Measuring Protein Degradation with Green Fluorescent Protein.
G.L. Hager, Studying Nuclear Receptors with Green Fluorescent Protein Fusions.
Localization of Molecules:
M.R. Flory and T.N. Davis Localization of Calmodulin in Budding Yeast and Fission Yeast Using Green Fluorescent Protein.
K.R. Olson and J.B. Olmsted, Analysis of Microtubule Organization and Dynamics in Living Cells Using Green Fluorescent Protein-MAP4 Chimeras.
V. Georget, B. Terouanne, J.-C. Nicholas and C. Sultan, Trafficking of the Androgen Receptor.
C.D. Webb and O. Resnekov, Use of Green Fluorescent Protein for Visualization of Cell-Specific Gene Expression and Subcellular Protein Localization in Bacillus subtilis.
L.S. Barak, J. Zhang, S.S.G. Ferguson and M.G. Caron, Signaling, Desensitization, and Trafficking of G Protein Coupled Receptors Revealed by Green Fluorescent Protein Conjugates.
G.A. Dabiri, J.C. Ayoob, K.K. Turnacioglu, J.M. Sanger and J.W. Sanger, Use of Green Fluorescent Proteins Linked to Cytoskeletal Proteins to Analyze Myofibrillogenesis in Living Cells.
L. Lybarger and R. Chervenak, Use of Fluorescent Proteins in Single and Multicolor Flow Cytometry.
L. Lybarger and R. Chervanak, Flow Cytometric Analysis of Transcription: Use of Green Fluorescent Protein Variants to Control Transfection Efficiency.
Y. Fang, C.-C. Huang, S.R. Kain and X. Li, Use of Co-expressed Enhanced Green Fluorescent Protein as a Marker for Identifying Transfected Cells.
L.A.C. Blair, K.K. Bence and J. Marshall, The Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second Messenger Signaling in Neurons.
A. Miyawaki, J.M. Matheson, L.G. Sayers, A.Muto, T. Michikawa, T. Furuichi and K. Mikoshiba, Expression of Green Fluorescent Protein and Inositol 1,4,5-Trisphosphate Receptor in Xenopus laevis Oocytes.
T. Takada, K. Yoshida, K. Nakamura, G. Tsujimoto, M. Katsuki and S. Sugano, Expression of Green Fluorescent Protein in Transgenic Mice.
A.S. Verkman, Green Fluorescent Protein as a Probe to Study Intracellular Solute Diffusion.
T. Aoki, K.S. Koch, H.L. Leffert and H. Watabe, Application of Green Fluorescent Protein-Protein A Fusion Protein to Western Blotting.
P. Prabhakara, M. Kallio and J.C. Herr, Green Fluorescent Protein As a Reporter for Promoter Analysis of Testis-Specific Genes in Transgenic Mice.
L.E. Bermudez, F.J. Sangari and A. Parker, Use of Green Fluorescent Protein for the Measurement of Bacteria-Host Interactions.
D.W. Galbraith, Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein.
P.E. Urwin, S.G. Moller, J.K. Blumsom and H.J. Atkinson, Continual Green Fluorescent Protein Monitoring of Promoter Activity in Plants.
J.P. Levy, R.R. Muldoon, C.J. Link, Jr. And S.R. Kain, Retroviral Expression of Green Fluorescent Protein.
J.J. Lemasters, D.R. Trollinger, T. Qian, W.E. Cascio and H. Ohata, Confocal Imaging of Ca2+, pH, Electrical Potential and Membrane Permeability in Single Living Cells.
J.P. Levy, R.R. Muldoon, I.A. Mazo, S.R. Kain and C.J. Link, Jr., In Vivo Retroviral Transduction and Expression of Green Fluorescent Protein.
Mutants and Variants of Green Fluorescent Protein:
Y. Kimata, C. Ren Lim and K. Kohno, S147P Green Fluorescent Protein: A Less Thermosensitive Green Fluorescent Protein Variant.
G.J. Palm and A. Wlodawer, Spectral Variants of Green Fluorescent Protein.
L.A. King, C.J. Thomas, N. Wilkinson and R.D. Possee, Expression of Green Fluorescent Protein Using Baculovirus Vectors.
R. Heim, Green Fluorescent Protein Forms for Energy Transfer.
C.J. Link, Jr., S. Wang, R.R. Muldoon, T. Seregina and J.P. Levy, Use of Codon Modified, Red-shifted Variants of Green Fluorescent Protein Genes to Study Viral Mediated Gene Transfer.
X. Zhao, X. Jiang, C.-C. Huang, S.R. Kain and X. Li, Generation of a Destabilized Form of Enhanced Green Fluorescent Protein.
S. Inouye, K. Umesono, and F.I. Tsuji, Spectral Properties of Green Fluorescent Protein-S65A.
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