Glycobiology Protocols
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Glycobiology Protocols

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Description

Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell.
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Product details

  • Hardback | 382 pages
  • 158 x 234 x 26mm | 739.37g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2007 ed.
  • 112 Illustrations, black and white; XVIII, 382 p. 112 illus.
  • 1588295532
  • 9781588295538

Review quote

"...a very valuable handbook for a wide audience of specialists involved in studies of carbohydrates and carbohydrate-containing biopolymers; glycobiologists, biochemists, bioorganic chemists, and molecular and cell biologists." -Biochemistry
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Table of contents

The Application of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues
Ningguo Gao

Partial Purification of Mannosylphosphorylundecaprenol Synthase from Micrococcus luteus: A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products
Jeffrey S. Rush and Charles J. Waechter

Assay of Dolichyl-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix
John S. Schutzbach

O-Mannosylation in Mammalian Cells
Tamao Endo and Hiroshi Manya

Methods for Analysis of Unusual Forms of O-glycosylation
Aleksandra Nita-Lazar and Robert S. Haltiwanger

Structural Analysis of Naturally Occurring Sialic Acids
Johannis P. Kamerling and Gerrit J. Gerwig

Non-Radioactive Trans-Sialidase Screening Assay
Silke Schrader and Roland Schauer

Structural Determination of O-Glycans by Tandem Mass Spectrometry
Catherine Robbe, Jean-Claude Michalski, and Calliope Capon

Detailed Structural Analysis of N-glycans Released from Glycoproteins in SDS-PAGE Gel Bands Using HPLC Combined with Exoglycosidase Array Digestions
Louise Royle, Catherine M. Radcliffe, Raymond A. Dwek, and Pauline M. Rudd

Molecular Modelling of Glycosyltransferases
Anne Imberty, Michaela Wimmerova, Jaroslav Koca, and Christelle Breton

b-Galactoside a2,6 Sialyltransferase (ST6Gal.I) and the Sialyl a2,6-galactosyl-linkage in Tissues and Cell Lines
Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo

Visualizing Intracellular Distribution and Activity of Core 2 b(1,6)N-acetylglucosaminyltransferase-I (C2GnT-I) in Living Cells
Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari

Gene Expression Analysis of Glycosylation-Related Genes by Real-Time PCR
Juan J. Garcia-Vallejo, Sonja I. Gringhuis, Willem van Dijk, and Irma van Die

Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells
Inka Brockhausen, Xiaojing Yang, and Mark Harrison

Micromethods for the Characterization of Lipid A-Core and O Antigen Lipopolysaccharide
Cristina L. Marolda, Piya Lahiry, Enrique Vines, Soledad Saldias, and Miguel A. Valvano

Assays for a Galactosyltransferase Involved in the Assembly of the O7 Antigen Repeat Unit of Escherichia coli
Inka Brockhausen, Walter A. Szarek, John G. Riley, and Jason Vlahakis

High Through-Put Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Proximity Assay Utilizing Binding to Cationic Dyes
Karen J. Rees-Milton and Tassos P. Anastassiades

Quantitative Analysis of Mucins in Mucosal Secretions Using Indirect ELISA
Pablo Argueso and Ilene K. Gipson

Molecular Dissection of the Mouse Zona Pellucida: An Electron Microscopic Perspective Utilizing High Resolution Colloidal-Gold Labeling Methods
Frederick W.K. Kan

Soluble Adamantly GSL Analogues as Probes of GSL Function
Clifford Lingwood, Skanda Sadacharan, Maan Abul-Milh, Murugespillai Mylvaganum, and Marcus Peter

Diagnosis of Krabbe Disease by Use of a Natural Substrate
John W. Callahan and Marie-Anne Skomorowski

In Vitro Assays of the Functions of Calnexin and Calreticulin, Lectin-Chaperones of the Endoplasmic Reticulum
Breanna S. Ireland, Monika Niggemann, and David B.Williams

Quantitative Measurement of Selectin-Ligand Interactions: Assays to Identify a Sweet Pill in a Library of Carbohydrates
Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta

Sialic Acid Binding Specificities of Siglecs
Nadine Bock and Soerge Kelm
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