Gene Expression Technology

Gene Expression Technology

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Description

The aim of this volume is to give recombinant DNA researchers the most up-to-date knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells. The methods presented should enable the reader to design effective strategies for the expression of cloned genes and cDNAs, and prove useful in solving the majority of expression problems that are likely to be encountered.
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Product details

  • Paperback | 681 pages
  • 196.85 x 254 x 25.4mm | 1,043.26g
  • Academic Press Inc
  • San Diego, United States
  • English
  • New edition
  • New edition
  • indexes
  • 012287045X
  • 9780122870453

Table of contents

Expression in escherichia coli; promoters; translation; proteases; gene fusions; secretion; protein refolding; expression in bacillus subtilis; intracellular production; inducible expression of regulatory proteins; expression in yeast; plasmid vectors; inducible expression cassettes; constitutive expression cassettes; translation; proteases; protein secretion and modification; expression in mammalian cells; transfection methods; markers for selection and amplification; growth of cell lines; posttranslation processing, modification, and secretion; mutagenesis.
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Review quote

"This volume should prove invaluable to investigators with a molecular biology background who need to express a given gene but are not particularly experienced in this area. Many of the contributors have been able to effectively communicate years worth of experience and unpublished observations in what often was a black magic science. This volume clearly shows that things have changed and that the methodlolgy is now in the hands of the skilled non-expert." AMERICAN JOURNAL OF PHYSIOLOGY
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