Gene Delivery to Mammalian Cells: Viral Gene Transfer Techniques Volume 2
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Gene Delivery to Mammalian Cells: Viral Gene Transfer Techniques Volume 2

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The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology-assessment of gene function and gene therapy-require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.show more

Product details

  • Hardback | 566 pages
  • 157.5 x 231.1 x 30.5mm | 907.2g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2004.
  • biography
  • 1588290956
  • 9781588290953

Review quote

For Volumes 1 & 2 "This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology "...clearly and succinctly presented...a must for any biology or medical library." - ASM Newsshow more

Back cover copy

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.show more

Table of contents

Part I. Delivery Using Adenoviruses Adenovirus-Mediated Gene Delivery: An Overview Joanne T. Douglas DNA Delivery to Cells in Culture: Generation of Adenoviral Libraries for High-Throughput Functional Screening Miroslava Ogorelkova, Seyyed Mehdy Elahi, David Gagnon, and Bernard Massie Adenovirus-Mediated Gene Delivery to Skeletal Muscle Joanne T. Douglas Delivery of Adenoviral DNA to Mouse Liver Sheila Connelly and Christine Mech Delivery of DNA to Lung Airway Epithelium Daniel J. Weiss Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors Paul N. Reynolds Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors Joseph M. Alisky and Beverly L. Davidson. Adenovirus-Mediated Gene Transfer to Tumor Cells Manel Cascallo and Ramon Alemany Adenovirus-Mediated Gene Delivery to Dendritic Cells Laura Timares, Joanne T. Douglas, Bryan W. Tillman, Victor Krasnykh, and David T. Curiel Part II. Delivery Using Adeno-Associated Viruses Overview of Adeno-Associated Viral Vectors Thomas M. Daly AAV Vector Delivery to Cells in Culture Andrew Smith, Roy Collaco, and James P. Trempe AAV-Mediated Gene Transfer to Skeletal Muscle Roland W. Herzog AAV-Mediated Gene Transfer to the Liver Thomas M. Daly. AAV-Mediated Gene Transfer to Mouse Lungs, Christine L. Halbert and A. Dusty Miller Gene Delivery to the Mammalian Heart Using AAV Vectors Danny Chu, Patricia A. Thistlethwaite, Christopher C. Sullivan, Mirta S. Grifman, and Matthew D. Weitzman Gene Delivery to the Mouse Brain with Adeno-Associated Virus Marco A. Passini, Deborah J. Watson, and John H. Wolfe Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus Selvarangan Ponnazhagan and Frank Hoover Gene Delivery toHuman and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors Arun Srivastava Part III. Delivery Using Herpes Simplex Viruses Delivery Using Herpes Simplex Virus: An Overview William F. Goins, Darren Wolfe, David M. Krisky, Qing Bai, Ed A. Burton, David J. Fink, and Joseph C. Glorioso Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors Baohong Cao and Johnny Huard Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System James R. Goss, Atsushi Natsume, Darren Wolfe, Marina Mata, Joseph C. Glorioso, and David J. Fink Gene Transfer to Glial Tumors Using Herpes Simplex Virus Ajay Niranjan, Darren Wolfe, Wendy Fellows, William F. Goins, Joseph C. Glorioso, Douglas Kondziolka, and L. Dade Lunsford Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells Darren Wolfe, James B. Wechuck, David M. Krisky, Julie P. Goff, William F. Goins, Ali Ozuer, Michael E. Epperly, Joel S. Greenberger, David J. Fink, and Joseph C. Glorioso Part IV. Delivery Using Baculoviruses Baculovirus-Mediated Gene Delivery into Mammalian Cells Raymond V. Merrihew, Thomas A. Kost, and J. Patrick Condreay Part V. Delivery Using Lentiviruses Gene Delivery by Lentivirus Vectors: An Overview Tal Kafri Lentiviral Vectors for the Delivery of DNA into Mammalian Cells Roland Wolkowicz, Garry P. Nolan, and Michael A. Curran Stable Gene Delivery to CNS Cells Using Lentiviral Vectors Deborah J. Watson, Brian A. Karolewski, and John H. Wolfe Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors Hiroyuki Miyoshi Delivery of Genes to the Eye Using Lentiviral Vectors Masayo Takahashi Lentiviral Transduction of Human Dendritic Cells Roland Schroers and Si-Yi Chen Part VI. Delivery Using Retrovirusesshow more