Gene Delivery to Mammalian Cells: Nonviral Gene Transfer Techniques v. 1
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Gene Delivery to Mammalian Cells: Nonviral Gene Transfer Techniques v. 1

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Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.show more

Product details

  • Hardback | 301 pages
  • 152.4 x 228.6 x 25.4mm | 628.22g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • biography
  • 1588290867
  • 9781588290861

Review quote

For Volumes 1 & 2 "This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology Volume 1 "...provides insights into the choices available in this important area of biology and medicine." - Microbiology Todayshow more

Back cover copy

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.show more

Table of contents

Part I. Delivery Using Chemical Methods Chemical Methods for DNA Delivery: An Overview Dexi Liu, Evelyn F. Chiao, and Hui Tian Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran Gregory S. Pari and Yiyang Xu DNA Delivery to Cells in Culture Using Peptides Lei Zhang, Nicholas Ambulos, and A. James Mixson DNA Delivery to Cells in Culture Using PNA Clamps Todd D. Giorgio and Shelby K. Wyatt Dendrimer-Mediated Cell Transfection In Vitro James R. Baker, Jr., Anna U. Bielinska, and Jolanta F. Kukowska-Latallo DNA Delivery to Cells in Culture Using Cationic Liposomes Shelby K. Wyatt and Todd D. Giorgio Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium John Marshall, Nelson S. Yew, and Seng H. Cheng Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium Young K. Song, Guisheng Zhang, and Dexi Liu Delivery of DNA to Tumor Cells Using Cationic Liposomes Duen-Hwa Yan, Bill Spohn, and Mien-Chie Hung Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes Lalitha R. Belur and R. Scott McIvor Part II. Delivery Using Physical Methods Gene Delivery Using Physical Methods: An Overview Te-hui W. Chou, Subhabrata Biswas, and Shan Lu Gene Delivery to Mammalian Cells by Microinjection Robert King Delivery of DNA to Cells in Culture Using Particle Bombardment William C. Heiser Delivery of DNA to Skin by Particle Bombardment Shixia Wang, Swati Joshi, and Shan Lu Biolistic Transfection of Cultured Organotypic Brain Slices A. Kimberley McAllister Efficient Electroporation of Mammalian Cells in Culture Peter A. Barry Delivery of DNA to Skin by Electroporation Nathalie Dujardin and Veronique Preat In VivoDNA Electrotransfer in Skeletal Muscle Guenhael Sanz, Saulius Satkauskas, and Lluis M. Mir Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo Mark J. Jaroszeski, Loree C. Heller, Richard Gilbert, and Richard Heller Hydrodynamic Delivery of DNA Joseph E. Knapp and Dexi Liu Naked DNA Gene Transfer in Mammalian Cells Guofeng Zhang, Vladimir G. Budker, James J. Ludtke, and Jon A. Wolff Microparticle Delivery of Plasmid DNA to Mammalian Cells Mary Lynne Hedley and Shikha P. Barman DNA Delivery to Cells in Culture Using Ultrasound Thomas P. McCreery, Robert H. Sweitzer, and Evan C. Unger DNA Delivery to Cells In Vivo by Ultrasound Thomas P. McCreery, Robert H. Sweitzer, Evan C. Unger, and Sean Sullivan Indexshow more