Footprinting of Nucleic Acid-Protein Complexes

Footprinting of Nucleic Acid-Protein Complexes

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"Separation, Detection and Characterization of Biological Macromolecules" is a series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods and for practical benchtop use. "Footprinting of Nucleic Acid-Protein Complexes" provides protocols for studying the stoichiometry, binding site size and location, and structural changes in nucleic acids caused by their interaction with proteins. The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments "in vivo" and "in vitro", in eukaryotes and in prokaryotes, at qualitative and quantitative levels and across many disciplines. This book is aimed at bench scientists, from graduate students on, and should be of value in industrial labs as well as in academic settings. Use of different footprinting approaches can provide insights into DNA-protein systems.
The protocols contained in this book are written to be "user-friendly" and thus should be conducive to extending the use of footprinting to new systems. The section on quantitative analysis of DNAse I footprints should prove especially useful for in-depth evaluation of cooperative interactions.
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Product details

  • Spiral bound | 216 pages
  • 196.85 x 234.95 x 12.45mm | 498.95g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0125865007
  • 9780125865005

Table of contents

Quantitative DNAse I footprinting, M. Brenowitz et al; footprinting DNA-protein and RNA-ligand interactions using the chemical nucleolytic activity of 1, 10 phenanthroline-copper, A. Mazumder; hydroxyl radical footprinting, J.S. Bashkin and T.D. Tullius; permanganate probing, J.D. Gralla et al; photofootprinting DNA "in vitro" and "in vivo", M.M. Becker; exonuclease III digestion, J. Cao and A. Revzin; interface and missing contact footprinting, J. Cao and A. Revzin.
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