Fluorescent and Luminescent Probes for Biological Activity

Fluorescent and Luminescent Probes for Biological Activity : A Practical Guide to Technology for Quantitative Analysis

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This book provides both new and existing scientists with a head-start into the practice of optical probe technology, the problems, the pitfalls and limitations likely to be encountered, the application to practical biological questions and the likely areas for future development. The book focuses on the practical hardware requirements and underlying principles of operation, the strategies leading to development of optical probes and their application to living material.
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Product details

  • Spiral bound | 464 pages
  • 195.58 x 256.54 x 20.32mm | 793.78g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0124778305
  • 9780124778306

Table of contents

Fluorescence microscopy, J.S. Ploem; introduction to fluorescent probes - properties, history and applications, F.H. Kasten; intracellular ion indicators, R. Haughland; radox confocal imaging - intrinsic fluorescent probes of cellular metabolism, B. Chance and B.R. Masters; bio- and chemi-luminescent indicators for molecular signalling and function in living cells, A.K. Campbell and G. Sala-Newby; acridine orange as a probe for molecular biology, Alexander Aelenin; fluorescent lipid analogues applications in cell and membrane biology, J.W. Kok and D. Hoekstra; probes for endoplasmic reticulum, M. Terasaki; probing mitochondrial membrane potential in living cells by a J-aggregate forming dye, L.B. Chen and S.T. Smiley; optical probes for cyclic AMP, S.R. Adams, et al; potentiometric membrane dyes, L.M. Loew; real time fluorescence ratio imaging in living cells, W.T. Manson; a small area-cooled-ccd camera and software for fluorescence ratio imaging, G. Law and W. O'Brien; multiparamater imaging of cellular function, G. Bright; digital image analysis - software approaches and applications, G. Relf; fluorescent probes in practice - potential artefacts, J. Hoyland; confocal microscopy principles, practice and options, C.J.R. Sheppard; confocal ramam microspectrosopy, G.J. Puppels, et al; dual excitation confocal fluorescent microscopy, I. Kurtz; properties of low light level video frame rate cameras, P. Tomkins and A. Lyons; properties of low light level slow scan detectors, R. Aikens; fast photometric measurements of cell function combined with electrophysioogy, J. Dempster; flow cytometry - use of multiparameter kinetics to evaluate several activation parameters simultaneously in individual living cells, E.R. Simons; flow cytometric analysis and sorting of viable cells, J.F. Keij and H. Herweijer; introducing and calibrating probes in cells and organelles, D. Williams, et al; photolabila caged compounds, A.M. Gurney; fluorescent analogs - optical biosensors of the chemical and molecular dynamics of macromolecules in living cells, K.M. Hahn, et al; fluorescence and luminescence techiques to probe ion activities in living plant-cells,, M. Tester, et al; assessment of gap junctional inter-cellular communication in living cells using fluorescence techniques, J.E. Trosko, et al; fast multiste optical measurement of membrane potential, J-Y Wu and L.B. Cohen; ohotoactivation of fluorescence as a probe for cytoskeletal dynamics in mitosis and cell motiligy, K.E. Sawin, et al; video imaging of lipid order, K. Florine-Cateel, et al.
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