Exosomes and Microvesicles

Exosomes and Microvesicles : Methods and Protocols

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Description

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.show more

Product details

  • Hardback | 228 pages
  • 178 x 254 x 14.22mm | 652g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 1st ed. 2017
  • 21 Tables, color; 21 Illustrations, color; 18 Illustrations, black and white; XII, 228 p. 39 illus., 21 illus. in color.
  • 1493967266
  • 9781493967261
  • 792,490

About Andrew F. Hill

Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute, Email: a.hill@unimelb.edu.aushow more

Back cover copy

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.show more

Table of contents

Preface... Table of Contents... Contributing Authors... 1. Methods to Analyze EVsBernd Giebel and Clemens Helmbrecht2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular VesiclesSybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij3. Immuno-Characterization of Exosomes using Nanoparticle Tracking AnalysisKym McNicholas and Michael Z. Michael4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron MicroscopyRomain Linares, Sisareuth Tan, Celine Gounou, and Alain R. Brisson5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCRShayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM (TM) SystemLesley Cheng and Andrew F. Hill7. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal UltrafiltrationRong Xu, Richard J. Simpson, and David W. Greening8. Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)Rikke Baek and Malene Moller Jorgensen9. Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic ActivationKarine Laulagnier, Charlotte Javalet, Fiona Hemming, and Remy Sadoul10. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular SpaceR. Perez-Gonzalez, S.A. Gauthier, A. Kumar, M. Saito, M. Saito, and E. Levy11. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion TransmissionPascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graca Raposo and Didier Vilette12. Isolation of Platelet-Derived Extracellular VesiclesMaria Aatonen, Sami Valkonen, Anita Boing, Yuana Yuana, Rienk Nieuwland and Pia Siljander13. Bioinformatics Tools for Extracellular Vesicles ResearchShivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan14. Preparation and Isolation of siRNA-Loaded Extracellular VesiclesPieter Vader, Imre Mager, Yi Lee, Joel Z. Nordin, Samir EL Andaloussi, and Matthew J.A. Wood15. Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow ConditionsS.M. van Dommelen, M. Fish, A.D. Barendrecht, R.M. Schiffelers, O. Eniola-Adefeso, P. Vadershow more