Eukaryotic Transcription Factors

Eukaryotic Transcription Factors

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Description

Now in two-colour throughout, the fourth edition of Eukaryotic Transcription Factors has been completely rewritten and restructured to take into account the tremendous advances in our understanding of transcription factors and the mechanisms by which they act. Considerable emphasis has been given to the interaction between transcription factors and chromatin structure. Also included is an entirely new section on the mediator complex and expansion of the space devoted to co-activators and co-repressors.

This book is essential reading for all those who wish to understand the impact of molecular biology on medicine, whatever their speciality.
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Product details

  • Hardback | 384 pages
  • 196 x 250 x 22mm | 1,061.4g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 4th edition
  • Approx. 300 illustrations
  • 0124371787
  • 9780124371781

Table of contents

List of Tables
DNA sequences, transcription factors and chromatin structure
The importance of transcription
Chromatin structure and its remodelling
Chromatin structure and gene regulation
Chromatin remodelling factors
Histone acetylation
DNA sequence elements
The gene promoter
Sequences involved in the basic process of transcription
Sequences involved in regulated transcription
Sequences which act at a distance
Negatively acting DNA sequences
Interaction between factors bound at various sites
Conclusions
References
Methods for studying transcription factors
Introduction
Methods for studying DNA-protein interactions
DNA mobility shift assay
DNaseI footprinting assay
Methylation interference assay
In vivo Footprinting assay
Methods for purifying and/or cloning transcription factors
Protein purification
Gene cloning
Use of cloned genes
Domain mapping of transcription factors
Determining the DNA binding specificity of an uncharacterised factor
Identification of target genes for transcription factors
Conclusions
References
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About David S. Latchman

David S. Latchman graduated with a BA (First Class Honours) in Natural Sciences from Cambridge University and subsequently obtained a PhD in Genetics from Cambridge University. Following a period of post-doctoral research at Imperial College London, he was appointed to a lectureship at University College London in 1984. Subsequent appointments at UCL included Director of the Medical Molecular Biology Unit (1988), Professor of Molecular Pathology and Head of the Department of Molecular Pathology (1991), Director of the Windeyer Institute of Medical Sciences (1996) and in 1999, Dean of the Institute of Child Health and Professor of Human Genetics. At the beginning of 2003, he took up a new appointment as Master of Birkbeck, University of London, leading this multi-faculty College comprising over 1,000 staff and 16,000 students studying at levels from short courses to PhDs. He continues to retain an active research laboratory at the Institute of Child Health, UCL and in recognition of this he is now Professor of Genetics at Birkbeck and UCL. Since he set up his own laboratory in 1984, Professor Latchmans interests have focused particularly on the regulation of gene expression in mammalian cells by specific transcription factors. Initial studies, involved the eukaryotic virus Herpes Simplex Virus (HSV) and were aimed at elucidating why this virus was able to replicate in epithelial cells, whilst establishing silent latent infections in neuronal cells. The identification of a cellular POU family transcription factor, Oct-2, which was expressed in neuronal cells and blocked the virus-lytic cycle, led to an interest in the role of specific POU family transcription factors in regulating cellular as well as viral gene expression in neuronal cells. As well as studies of the role of Oct-2, these investigations resulted in the characterisation of a small sub-family of POU family transcription factors which comprised three members: Brn-3a, Brn-3b and Brn-3c. In particular, the key role of Brn-3a in promoting neuronal differentiation and enhancing the survival of neuronal cells was defined by Professor Latchmans laboratory and has important therapeutic potential for the treatment of human neurodegenerative diseases involving losses of neuronal cells. More recently, Professor Latchmans group has also studied the role of specific transcription factors in the heart and in particular, in its response to damage caused by ischaemia, as in a heart attack. They have demonstrated that a specific transcription factor STAT-1 becomes activated during ischaemia and induces a number of genes which enhance the programmed cell death of cardiac cells, resulting in the loss of these irreplaceable cells. Thus, inhibition of STAT-1 represents a potential therapeutic mechanism for decreasing the damage caused by interruption of blood flow to the heart. On the basis of his scientific research, Professor Latchman was awarded a DSc by London University in 1994 and was appointed a Fellow of the Royal College of Pathologists (FRCPath) in 1999. He serves on a number of Committees, including the Department of Health, Genetics and Insurance Committee, the National Biological Standards Board and its Scientific Policy Advisory Committee, the Health Protection Agency and the Research Strategy Committee of the Higher Education Funding Council for England.
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