Enzymology at the Membrane Interface: Intramembrane Proteases: Volume 584

Enzymology at the Membrane Interface: Intramembrane Proteases: Volume 584

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Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.show more

Product details

  • Hardback | 494 pages
  • 152 x 229 x 26.92mm | 930g
  • Elsevier Science Publishing Co Inc
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0128122137
  • 9780128122136

Table of contents

Biochemical Characterization of Function and Structure of RseP, an Escherichia Coli S2P ProteaseY. Hizukuri, K. Akiyama and Y. AkiyamaSignal Peptidase Enzymology and Substrate Specificity ProfilingR.E. Dalbey, D. Pei and OE.D. EkiciScreening and Characterization Strategies for Nanobodies Targeting Membrane ProteinsS. Veugelen, M. Dewilde, B.D. Strooper and L. Chavez-GutierrezProbing the Activity of Eukaryotic Rhomboid Proteases in VitroB. Cordier and M.K. LembergExpression, Purification, and Enzymatic Characterization of Intramembrane ProteasesR. Zhou, Y. Shi and G. YangAnalyzing Amyloid-ss Peptide Modulation Profiles and Binding Sites of γ-Secretase ModulatorsJ. Trambauer, A. Fukumori, B. Kretner and H. SteinerProbing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility MethodT. TomitaA New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman SpectroscopyJ.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi and I. Ubarretxena-BelandiaAn Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the MembraneR.P. Baker and S. UrbanProduction of Recombinant Rhomboid ProteasesE. Arutyunova, R. Panigrahi, K. Strisovsky and M.J. LemieuxMechanism and Inhibition of Rhomboid ProteasesK. StrisovskyEnzymatic Assays for Studying Intramembrane ProteolysisD.M. Bolduc, D.J. Selkoe and M.S. WolfeMethods for Structural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Super FamilyY. Yang, N. Ke, S. Liu and W. LiFunctional Study of the Vitamin K Cycle Enzymes in Live CellsJ.-K. Tie and D.W. StaffordActivity Assays for Rhomboid ProteasesE. Arutyunova, K. Strisovsky and J. Lemieuxshow more

About Michael H. Gelb

Michael H. Gelb studied chemistry and biochemistry as an undergraduate at the University of California at Davis. His Ph.D. studies with Stephen G. Sligar at Yale University led to a better understanding of the catalytic mechanism of cytochrome P450. As an American Cancer Society Postdoctoral Fellow in the laboratory of the late Robert H. Abeles at Brandeis University, Gelb studied a variety of mechanism-based inactivators of serine proteases and developed fluorinated ketones as tight-binding inhibitors of several classes of proteases. In 1985 Gelb became a faculty member in the Departments of Chemistry and Biochemistry at the University of Washington. Major breakthroughs in the group include the development of methods to properly analyze the action of enzymes on membrane surfaces, the discovery of protein prenylation (farnesylation and geranylgeranylation) in mammalian cells (together with John A. Glomset), the development of Isotope-Coded Affinity Tags (ICAT reagents) for proteomic applications (together with Ruedi Aebersold), and the development of newborn screening for lysosomal storage diseases by mass spectrometry.show more