Enzyme Kinetics and Mechanism: Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates Detection and Characterization of Enzyme Reaction Intermediates: Pt. F Volume 354 : Enzyme Kinetics and Mechanism : Detection and Characterization of Enzyme Reaction Intermediates
- Hardback | 650 pages
- 160 x 237.7 x 26.7mm | 839.16g
- 04 Nov 2002
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
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Table of contents
Rapid Mix-Quench MALDI-TOF Mass Spectrometry for Analysis of Enzymatic Systems.
Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing Techniques.
Trapping of ï ¡-Glycosidase Intermediates.
Trapping Covalent Intermediates on ss-Glycosidases.
2-Hydroxy-6-keto-nona-2,4-diene 1,9-Dioic Acid 5,6-Hydrolase: Evidence from18O Isotope Exchange for gem-Diol Intermediate.
Nucleoside-Diphosphate Kinase: Structural and Kinetic Analysis of Reaction Pathway and Phosphohistidine Intermediate.
Galactose-1-Phosphate Uridylyltransferase: Kinetics of Formation and Reaction of Uridylyl-Enzyme Intermediate in Wild-Type and Specifically Mutated Uridylyltransferases.
Kinetic Evidence for Covalent Phosphoryl-Enzyme Intermediate in Phosphotransferase Activity of Human Red Cell Pyrimidine Nucleotidases.
Characterization of ï ¡(2->6) -Sialyltransferase Reaction Intermediates: Use of Alternative Substrates to Unmask Kinetic Isotope Effects.
Use of Sodium Borohydride to Detect Acyl-Phosphate Linkages in Enzyme Reactions.
Evidence for Phosphotransferases Phosphorylated on Aspartate Residue in N-Terminal DXDX(T/V) Motif.
MurC and MurD Synthetases of Peptidoglycan Biosynthesis: Borohydride Trapping of Acyl-Phosphate Intermediates.
Transaldolase B: Borohydride Reduction Trapping of Schiff Base Intermediate between Dihydroxyacetone and ï -Amino Group of Active-Site Lysine Residue.
Use of NMR and Borohydride Trapping to Provide Evidence for Covalent Enzyme-Substrate Imine Intermediate.
Detection of Covalent Tetrahedral Adducts by Differential Isotope Shift (1)(3)C NMR: Acetyl-Enzyme Reaction Intermediate Formed by 3-Hydroxy-3-methylglutaryl-CoA Synthase.
Detection of Intermediates in Reactions Catalyzed by PLP-Dependent Enzymes: O-Acetylserine Sulfhydrylase and Serine-Glyoxalate Aminotransferase.
Protein Tyrosine Phosphatase: X-Ray Crystallographic Observation of Cysteinyl-Phosphate Reaction Intermediate.
GTP:GTP Guanylyltransferase: Trapping Procedures for Detecting and Characterizing Chemical Nature of Enzyme-Nucleotide Phosphoramidate Reaction Intermediate.
ï §-Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B.
ï §-Glutamyltranspeptidase and ï §-Glutamyl Peptide Ligases: Fluorophosphonate and Phosphonodifluoromethyl Ketone Analogs as Probes of Tetrahedral Transition State and ï §-Glutamyl-Phosphate Intermediate.
Stoichiometric Redox Titrations of Complex Metalloenzymes.
Urate Oxidase: Single-Turnover Stopped-Flow Techniques for Detecting Two Discrete Enzyme-Bound Intermediates.
Nitric Oxide Synthase: Use of Stopped-Flow Spectroscopy and Rapid-Quench Methods in Single-Turnover Conditions to Examine Formation and Reactions of Heme- O2 Intermediate in Early Catalysis.
Myeloperoxidase: Kinetic Evidence for Formation of Enzyme-Bound Chlorinating Intermediate.
Time-Resolved Resonance Raman Spectroscopy of Intermediates in Cytochrome Oxidase.
Porphobilinogen Deaminase: Accumulation and Detection of Tetrapyrrole Intermediates Using Enzyme Immobilization.
Adenosylcobalamin-Dependent Glutamate Mutase: Pre-Steady-State Kinetic Methods for Investigating Reaction Mechanism.
Ribonucleotide Reductase: Kinetic Methods for Demonstrating Radical Transfer Pathway in Protein R2 of Mouse Enzyme in Generation of Tyrosyl Free Radical.
Galactose Oxidase: Probing Radical Mechanism with Ultrafast Radical Probe.
Kinetic Characterization of Transient Free Radical Intermediates in Reaction of Lysine 2,3-Aminomutase by EPR Lineshape Analysis.
Demonstration of Peroxodiferric Intermediate in M-Ferritin Ferroxidase Reaction Using Rapid Freeze-Quench Moessbauer, Resonance Raman, and XAS Spectroscopies.
A Survey of Covalent, Ionic, and Radical Intermediates in Enzyme-Catalyzed Reactions.
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