Environmental Microbiology

Environmental Microbiology : Methods and Protocols

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The methods included in Environmental Microbiology: Methods and Pro- cols can be placed in the categories "Communities and Biofilms," "Fermented Milks," "Recovery and Determination of Nucleic Acids," and the review s- tion, containing chapters on the endophytic bacterium, Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, using the hemoglobin gene from a strain of Vitreoscilla 23 spp., and the use of chemical shift reagents and Na NMR to study sodium gradients in microorganisms, all of which should be of interest to investigators in these fields. The subjects treated within the different categories also cover a wide range, with methods ranging from those for the study of marine organisms, through those for the investigation of microorganisms occurring in ground waters, including subsurface ground waters, to other types of environmental waters, to as varied subjects as the biodiversity of yeasts found in northwest Argentina. The range of topics described in the Fermented Milks section is smaller, but significant for investigators in areas concerned with milk as an item of foods for infants, small children, and even adults.
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Product details

  • Hardback | 426 pages
  • 154.9 x 233.7 x 27.9mm | 793.8g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2004 ed.
  • XIV, 426 p.
  • 1588291162
  • 9781588291165

Back cover copy

Understanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments.
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Table of contents

Part I. Communities and Biofilms

Isolation and Molecular Characterization of Seawater Bacteria
Juliana M. Benito, Gustavo A. Lovrich, Faustino Sineriz, and Carlos M. Abate

Reverse Sample Genome Probing to Monitor Microbial Communities
E. Anne Greene and Gerrit Voordouw

T-RFLP Analysis: A Rapid Fingerprinting Method for Studying Diversity, Structure, and Dynamics of Microbial Communities
Werner Liesack and Peter F. Dunfield

Assessing Bacterial DNA Content in Aquatic Systems
Garrett W. Perney, Betsy R. Robertson, and D. K. Button

Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry
Mary Lowe, Alex Spiro, Anne O. Summers, and Joy Wireman

Molecular Characterization of Microbial Communities From Marine Environments
Nicolas G. Barengo, Juliana M. Benito, and Carlos M. Abate

Yeasts: Ecology in Northwest Argentina
Lucia I. C. de Figueroa, M. Alejandra Martinez, and John F. T. Spencer

Aeromonads in Environmental Waters
Anavella Gaitan Herrera

Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water
Mark E. Fuller

Bench Scale Flow Cell for Nondestructive Imaging of Biofilms
Eric S. Gilbert and Jay D. Keasling

Part II. Fermented Milks

a-Galactosidase Assay in Fermented Soymilk Products
Marisa S. Garro, Graciela Font de Valdez, and Graciela Savoy de Giori

Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteria
Maria Ines Torino and Graciela Font de Valdez

Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatography
Marisa S. Garro, Graciela Font de Valdez, and Graciela Savoy de Giori

Evaluation of Minimal Nutritional Requirements ofLactic Acid Bacteria Used in Functional Foods
Elvira M. Hebert, Raul R. Raya, and Graciela Savoy de Giori

Part III. Nucleic Acids, Recovery, and Determination

A Simple Method for Obtaining DNA Suitable for RAPD Analysis From Azospirillum
Raul O. Pedraza and Juan C. Diaz Ricci

PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates
M. Alejandra Martinez and Faustino Sineriz

A DNA-DNA Hybridization Method for the Detection and Quantification of Specific Bacterial Taxa in Natural Environments
Christopher E. Bagwell and Charles R. Lovell

13C and 1H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes
Anne-Marie Delort, Genevieve Gaudet, and Evelyne Forano

Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper Pulp Manufacturing
Ana Gutierrez, Jose C. del Rio, and Angel T. Martinez

Propolis: Chemical Micro-Heterogeneity and Bioactivity
Jose Domingos Fontana, Juliana Adelmann, Mauricio Passos, Marcelo Maraschin, Cristina A. de Lacerda, and Fernando Mauro Lancas

Enzymatic Saccharification of Cellulosic Materials
Luiz Pereira Ramos and Jose Domingos Fontana

Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater
Kazuya Watanabe, Natsuko Hamamura, and Nobuo Kaku

Identification of Copper-Resistant Microorganisms by PCR
Virginia H. Albarracin, Juliana M. Benito, Manuel Sineriz Louis, Maria J. Amoroso, and Carlos M. Abate

Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites
Liliana Villegas, Maria J. Amoroso, and Lucia I. C. de Figueroa

Medium for Differential Enumeration of Lactobacillus casei and Lactobacillus acidophilus From Lyophilized Mixed Cultures
Monica M. Locascio, Rosalia Alesso, Vilma I. Morata, and
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