Environmental Genomics

Environmental Genomics

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Description

Here is a manual for an environmental scientist who wishes to embrace genomics to answer environmental questions. The volume covers: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. This book provides a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations.
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Product details

  • Hardback | 364 pages
  • 140.21 x 222 x 16.51mm | 331.12g
  • Totowa, NJ, United States
  • English
  • 2008 ed.
  • 16 Tables, black and white; 63 Illustrations, black and white; XII, 364 p. 63 illus.
  • 1588297772
  • 9781588297778
  • 2,584,825

Back cover copy

Environmental genomics seeks to predict how an organism or organisms will respond, at the genetic level, to changes in their external environment. These genome responses are diverse and, as a result, environmental genomics must integrate molecular biology, physiology, toxicology, ecology, systems biology, epidemiology and population genetics into an interdisciplinary research program. Environmental Genomics serves as a manual for an environmental scientist who wishes to embrace genomics in an effort to answer environmental questions. The volume is divided into the sections: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. The studies presented will reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations. Environmental Genomics should aid researchers looking to reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations.
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Table of contents

TABLE OF CONTENTS

Methods in Molecular Biology - Environmental Genomics

Preface - C. Cristofre Martin

Section 1: Gene expression profiling

Chapters:

1. High-throughput Whole Mount in situ Hybridization of Zebrafish
Embryos for Analysis Tissue Specific Gene Expression Changes Following
Environmental Perturbation.
Louise E. Coverdale, Lindsay E. Burton, and C. Cristofre Martin

2. Fluorescent RNA Arbitrarily Primed (FRAP) PCR - A New Differential
Display Approach To Detect Contaminant-Induced Alterations Of Gene
Expression In Wildlife Species.
Doug Crump, Suzanne Chiu, Vance Trudeau & Sean Kennedy

3. Isolation of O3-response Genes from Arabidopsis thaliana using cDNA
Macroarray.
Masanori Tamaoki

4. Use Of cDNA Macroarrays And Gene Profiling For Detection Of Effects
By Environmental Toxicants.
Jason L. Blum, Melinda S. Prucha, Vishal J. Patel, and Nancy D.
Denslow

5. Constructing and Screening a cDNA library: Methods for
Identification and Characterization of Novel Genes Expressed Under
Conditions of Environmental Stress.
Kevin Larade and Kenneth B. Storey

6. Comparative Molecular Physiological Genomics: Heterologous probing
of cDNA arrays.
Sean F. Eddy and Kenneth B. Storey

7. Proteomic Analysis of Neuroendocrine Peptidergic System Disruption
Using the AtT20 Pituitary Cell Line as a Model.
Fumin Dong, Liming Ma, Michel Chretien and Majambu Mbikay

8 Proteomics Based Method For Risk Assessment Of Peroxisome
Proliferating Pollutants In The Marine Environment.
Susana Cristobal


9. Environmental Metabolomics using 1H NMR Spectroscopy.
Mark R. Viant
Section 2: Detection of whole genome mutation

10. Restriction Landmark Genome Scanning for the Detection of Mutations.
Jun-ichi Asakawa,

11. Use of the Comet Assay in Environmental Toxicology.
Loren Knopper and James P. McNamee

12. TheMicronucleus Assay Determination of Chromosomal Level DNA
Damage.
Michael Fenech

13. Fluorescence In Situ Hybridization (FISH) For The Detection Of
Chromosome Aberrations And Aneuploidy Induced By Environmental
Toxicants.
Francesca Pacchierotti and Antonella Sgura

14. Laboratory Methods For The Detection Of Chromosomal Structural
Aberrations In Human And Mouse Sperm By Fluorescence In Situ
Hybridization (FISH).
Francesco Marchetti, Debby Cabreros and Andrew J Wyrobek


Section 3: Determination of Species Diversity

15. Assembling DNA Barcodes: Analytical Protocols.
Jeremy R. deWaard, Natalia V. Ivanova, Mehrdad Hajibabaei and Paul D.N.
Hebert

16. Application of Suppressive Subtractive Hybridization to Uncover the
Metagenomic Diversity of Environmental Samples.
Elizabeth A. Galbraith, Dionysios A. Antonopoulos and Bryan A. White

17. 16S rRNA Targeted DGGE Fingerprinting Of Microbial Communicities.
Vesela A. Tzeneva, Hans G. H. J. Heilig, Wilma Akkermans van Vliet,
Antoon D. L. Akkermans, Willem M. de Vos, Hauke Smidt

18. An Emulsion PCR-Based Method for Molecular Haplotyping.
James G. Wetmur and Jia Chen
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Review quote

From the reviews:


"This book provides a thought-provoking selection of well-described and diverse techniques to apply to further our understanding of how organisms respond to the environment. ... The techniques are all accessible to a laboratory equipped for molecular biology and this book would provide an excellent addition to any environmental molecular biology laboratory book shelf, institutional library or individual researcher's collection." (Elizabeth Wellington and Emma Travis, Microbiology Today, November, 2008)
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