Diabetes Mellitus

Diabetes Mellitus

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Diabetes mellitus is the collective name for a group of diseases associated with hyperglycemia (high levels of blood glucose) caused by defects in insulin p- duction, insulin action, or both. About 6. 2% of the US population (17 million people) have diabetes mellitus. It is the leading cause of kidney failure, bli- ness, and amputations. It is also a major risk factor for heart diseases, stroke, and birth defects. Diabetes Mellitus: Methods and Protocols provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters cover a wide range of topics written by experts in their respective fields and are organized in two sections: Insulin Production and Insulin Action. The detailed experimental protocols presented, including the notes of interest, provide a very useful tool for basic researchers and clinicians for investigating and treating this disease. Each chapter starts with an introduction to a specific technique and explains its application in the field of diabetes research. Following the list of materials, a detailed description of the technique is presented in the methods section in a way that enables the successful execution of the protocol. The "Notes" section at the end discusses the pitfalls of the technique and alternative approaches. I am grateful to the numerous scientists who have contributed to this volume by writing both highly detailed and understandable chapters.
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Product details

  • Hardback | 192 pages
  • 158 x 232 x 20mm | 557.93g
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • X, 192 p.
  • 1588291480
  • 9781588291486
  • 2,616,733

Back cover copy

About 17 million Americans suffer from diabetes mellitus, a disease caused by defects in insulin production, insulin action, or both, and also a leading cause of kidney failure, blindness, and amputations. In Diabetes Mellitus: Methods and Protocols, leading researchers and clinical investigators describe their cutting-edge techniques for studying these defects at both the molecular and biochemical levels. Written in step-by-step detail to ensure ready reproducibility and robust results, these techniques allow investigators, both novices and those already active in the field, to study every major facet of insulin production and action. Each protocol includes an introduction to the technique, an explanation of its application, and a list of materials. Practical notes discuss how to avoid pitfalls, as well as how to adapt the methods to your own research.
Cutting-edge and highly practical, Diabetes Mellitus: Methods and Protocols provides basic scientists and clinical researchers, both experienced investigators and those new to the field, all the critical tools needed for the productive study of diabetes mellitus today.
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Table of contents

Part I. Insulin Production

Isolation of Islets of Langerhans from Rodent Pancreas
Colleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. Scarim

Purification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell Sorting
Geert Stange, Mark Van De Casteele, and Harry Heimberg

Assessment of Insulin Secretion in the Mouse
Marcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. Powers

Detection of Insulin Production by Immunohistochemistry
Susan C. Campbell and Wendy M. Macfarlane

Quantification of the Level of Insulin Gene Expression
Susan C. Campbell and Wendy M. Macfarlane

Chromatin Immunoprecipitation Using Isolated Islets of Langerhans
Marcelina Parrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge Ferrer

Adenoviral Gene Transfer into b-Cell Lines
Amber L. Mosley and Sabire OEzcan

Utilization of NOD Mice in the Study of Type 1 Diabetes
Dennis G. Karounos and Susan E. Goes

Part II. Insulin Action

Introduction of DNA into 3T3-L1 Adipocytes by Electroporation
Shuichi Okada, Masatomo Mori, and Jeffrey E. Pessin

Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes
Jaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire OEzcan

Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes
Jeffrey S. Elmendorf

Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay
John B. Knight and Ann Louise Olson

Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
Eileen L. Whiteman and Morris J. Birnbaum

Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity
Li-Ping Wang and Scott A. Summers

Assaying AKT/ Protein Kinase B Activity
Kyle L. Hoehn and Scott A.Summers

Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes
Daryll B. DeWald

Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes
Matthew J. Brady

Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle
Joseph T. Brozinick, Jr.

Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose Uptake
Maggie M-Y. Chi and Kelle H. Moley

Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters
Mary O. Carayannopoulos and Kelle H. Moley

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Review quote

"This book provides all the critical tools needed for the productive study of diabetes mellitus today." - Clinical Laboratory International

"Each chapter/protocol is well referenced and the book is indexed. Because Diabetes Mellitus: Methods and Protocols provides all the critical tools needed for the productive study of diabetes mellitus, it is a necessary purchase for libraries supporting basic research programs that study the molecular and biochemical bases of diabetes mellitus." -E-STREAMS
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