Developmental Hematopoiesis

Developmental Hematopoiesis : Methods and Protocols

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During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis-the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.show more

Product details

  • Hardback | 480 pages
  • 157.5 x 233.7 x 30.5mm | 816.48g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2005 ed.
  • 92 black & white illustrations, 4 colour illustrations, biography
  • 1588292967
  • 9781588292964

Back cover copy

Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys an array of methods available to study hematopoiesis and describes in detail the powerful techniques they have established to investigate embryonic, fetal, and (to a lesser extent) postnatal blood development. The authors apply their approaches to invertebrate (fruit flies) as well as vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Developmental Hematopoiesis: Methods and Protocols offers novice and seasoned researchers alike the critical tools needed to gain a fundamental understanding of stem cell biology-examining not only how hematopoietic stem cells form, but also how they differ in the embryo, the fetus, and the adult.show more

Table of contents

Part I. Genetic Approaches Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse Hanna K. A. Mikkola and Stuart H. Orkin Inducible Transgene Expression in Mouse ES Cells David T. Ting, Michael Kyba, and George Q. Daley Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells Hans-Willem Snoeck Part II. Analysis of Cell Fate Outcomes in Transplantation Models Flow Cytometric Analysis of Hematopoietic Development Edward F. Srour and Mervin C. Yoder Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flake Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice Scott A. Johnson and Mervin C. Yoder Part III. Animal Models Drosophila Hemocyte Development During Drosophila Embryogenesis Robert A. Schulz and Nancy Fossett Xenopus Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patient Fate Mapping Hematopoietic Lineages in the Xenopus Embryo Mary Constance Lane and Michael D. Sheets Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain) Yumi Izutsu and Mitsugu Maeno Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo James B. Turpen Zebrafish Analysis of Hematopoietic Development in the Zebrafish Noelle N. Paffett-Lugassy and Leonard I. Zon Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos Philippe Herbomel and Jean-Pierre Levraud. Chick In Vivo Methods to Analyze CellOrigins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo Francoise Dieterlen-Lievre, Sophie Creuzet, and Josselyne Salaun Mouse Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development Margaret H. Baron and Deanna Mohn Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo Catherine Robin and Elaine Dzierzak Hematopoietic Stem Cell Development During Mouse Embryogenesis Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godin Analysis of Hematopoietic Progenitors in the Mouse Embryo James Palis and Anne Koniski In Vivo and In Vitro Assays of Thymic Organogenesis Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburn Retroviral Transduction in Fetal Thymic Organ Culture Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors Helmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Mullner, and Hartmut Beug A New Clonal Assay System for Lymphoid and Myeloid Lineages Hiroshi Kawamoto and Yoshimoto Katsura Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang Analysis of the Vascular Potential of Hematopoietic Stem Cells Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scott Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture Elizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinson Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation Matthiasshow more