DNA Vaccines

DNA Vaccines : Methods and Protocols

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The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet "naked DNA," could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the "gene gun. " The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA. In some cases, protection against challenge with the pathogen was obtained. The latter result was - questionably the touchstone of a method of vaccination worthy of the name.
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Product details

  • Hardback | 529 pages
  • 157.5 x 231.1 x 33mm | 861.84g
  • Totowa, NJ, United States
  • English
  • 2000 ed.
  • XIX, 529 p.
  • 0896035808
  • 9780896035805

Back cover copy

In DNA Vaccines: Methods and Protocols, state-of-the-art review articles by leading experts summarize how to develop and employ the highly promising new DNA vaccines, what clinical results can be expected from their use, and what is known about how they work. Key topics range from vaccine design and construction to preparation and delivery methods, including the use of classical adjuvants, "genetic adjuvants," and the immunostimulatory properties of DNA and selected oligonucleotide sequences. Several contributors provide strategic ideas on antigen engineering and describe the particularly novel applications of DNA vaccine methodology that have recently emerged. In addition, there is a discussion of dendritic cells and antigen-presenting cells, the understanding of whose actions holds great promise for modulating the immune response, and thus for treating disease. Cutting-edge and comprehensive, DNA Vaccines: Methods and Protocols provides a broad panorama of the methods and thinking from which the vaccines of tomorrow will evolve, and so constitutes an invaluable sourcebook for both experts developing new applications and newcomers who want to gain mastery of the techniques and problems involved.
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Table of contents

Purification of Supercoiled Plasmid, Anthony P. Green. Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines, Joachim Schorr, Peter Moritz, and Martin Schleef. Development and Characterization of Lyophilized DNA Vaccine Formulations, Nancy L. Shen, Jukka Hartikka, Nancy A. Horn, Marston Manthorpe, and Magda Marquet. Repeated Use of Qiagen Columns in Large-Scale Preparation of Plasmid DNA, Derek Gregory, Ricardo E. Tascon, and Douglas B. Lowrie. The Immunology of DNA Vaccines, Thomas Tuting, Jonathan Austyn, Walter J. Storkus, and Louis D. Falo Jr. Methodology Used in DNA-Based Prophylactic and Therapeutic Immunization Against Hepatitis B Virus in Chimpanzees, Alfred M. Prince and Betsy Brotman. Intramuscular and Intradermal Injection of DNA Vaccines in Mice and Primates, Heather L. Davis. Veterinary DNA Vaccines, Sylvia van Drunen Littel-van den Hurk, Ralph P. Braun, and Lorne A. Babiuk. DNA-Based Immunization of Neonatal Mice, Cynthia L. Brazolot Millan and Heather L. Davis. Intramuscular Injection of DNA Vaccines in Fish, Joel Heppell and Heather L. Davis. Development of DNA Vaccines for Salmonid Fish, Eric D. Anderson and Jo-Ann C. Leong. CTL Analysis for Tumor Vaccines, Antonio Rosato, Gabriella Milan, Annalisa Zambon, Paola Zanovello, and Dino Collavo. The Use of Bone Marrow-Chimeric Mice in Determining the MHC Restriction of Epitope-Specific Cytotoxic T Lymphocytes, Akiko Iwasaki and Brian H. Barber. Immunostimulatory DNA Sequences: An Overview, John H. Van Uden and Eyal Raz. Immunostimulatory CpG Motifs and DNA Vaccines, Risini Weeratna, Arthur M. Krieg, and Heather L. Davis. In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors, Charles F. Reich and David S. Pisetsky. Adjuvants for Plasmid DNA Vaccines, Jon Norman, Jukka Hartikka, Pamela Strauch, and Marston Manthorpe. Cytokine and Costimulatory Factor-Encoding Plasmids as Adjuvants for DNA Vaccination, Kenji Okuda, Susumu Kawamoto, and Jun Fukushima. Optimizationof DNA Vaccines Through the Use of Molecular Adjuvants, Jong J. Kim and David B. Weiner. Cytokine Fusion Constructs as DNA Vaccines Against Tumors, Holden T. Maecker, Athanasia Syrengelas, and Ronald Levy. The Use of Conventional Immunologic Adjuvants in DNA Vaccine Preparations, Shin Sasaki and Kenji Okuda. Genetic Adjuvants, Hildegund C. J. Ertl, Susanna Pasquini, Zhenning He, Hongying Deng, Louise Showe, Wynetta Giles-Davis, Yijie Wang, InSug O, Hilary Marston, Magdalena Blaszczyk- Thurin, and Zhiquan Xiang. DNA Immunization in Combination with the Immunostimulant Monophosphoryl Lipid A, Donald L. Lodmell, Nancy B. Ray, and Larry C. Ewalt. Controlled Plasmid Delivery and Gene Expression: Applications for Nucleic Acid-Based Vaccines, Russell J. Mumper, Harry C. Ledebur, Jr., Alain P. Rolland, and Eric Tomlinson. Mucosal Immunization with DNA Vaccines, Michael J. McCluskie and Heather L. Davis. Preparations for Particle-Mediated Gene Transfer Using the Accell (R) Gene Gun, Michael D. Macklin, Robert J. Drape, and William F. Swain. Entrapment of Plasmid DNA Vaccines into Liposomes by Dehydration/Rehydration, Gregory Gregoriadis, Brenda McCormack, Mia Obrenovich, and Yvonne Perrie. DNA-Based Vaccination with Polycistronic Expression Plasmids, Reinhold Schirmbeck, Jan von Kampen, Karin Metzger, Jens Wild, Beate Gruner, Martin Schleef, Andrea Kroeger, Hansjoerg Hauser, and Joerg Reimann. A Nonviral Cytoplasmic T7 Autogene System and Its Applications in DNA Vaccination, Franck G. Sturtz, Yunsheng Li, Janine Shulok, H. Ralph Snodgrass, and Xiao-zhuo Chen. Immunization with Naked DNA Coexpressing Antigen and Cytokine via IRES, Jochen Heinrich, Bettina Strack, Michael Nawrath, Jovan Pavlovic, and Karin Moelling. Genetic Subunit Vaccines: A Novel Approach for Genetic Immunization, I. Frank Ciernik and David P. Carbone. Antigen Engineering in DNA Immunization, Shan Lu, Steve Manning, and James Arthos. Genetic Vaccination Targeting T-Cell Receptors, Alexis P. Godillot, Qiong
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Review quote

"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research

"The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters

"Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery

"Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science

"The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology

"Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today
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