DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA: Volume 212

DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA: Volume 212

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To understand the structure and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 211 of Methods of Enzymology, collate in one source all the methods and approaches needed to achieve this goal.
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Product details

  • Hardback | 501 pages
  • 152 x 229 x 25.4mm | 870g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0121821137
  • 9780121821135

Table of contents

Gel Electrophoresis and Topological Methods for Analysis of DNA Structure:
D.M. Crothers, J. Drak, J.D. Kahn, and S.D. Levene, DNA Bending, Flexibility, and Helical Repeat by Cyclization Kinetics.
S. Diekmann, Analyzing DNA Curvature in Polyacrylamide Gels.
D.M. Crothers and J. Drak, Global Features of DNA Structure by Comparative Gel Electrophoresis.
E.S. Abrams and V.P. Stanton, Jr. , Use of Denaturing Gradient Gel Electrophoresis to Study Conformational Transitions in Nucleic Acids.
R. Bowater, F. Aboul-Ela, and D.M.J. Lilley, Two-Dimensional Gel Electrophoresis of Circular DNA Topoisomers.
P. Dr~adoge and N.R. Cozzarelli, Topological Structures of DNA Knots and Catenanes.
Probing DNA Structure in Vitro:
D.M.J. Lilley, Probes of DNA Structure.
E. Palecek, Probing DNA Structure with Osmium Tetroxide Complexes in Vitro.
T. Kohwi-Shigematsu and Y. Kohwi, Detection of Non-B-DNA Structures at Specific Sites in Supercoiled Plasmid DNA and Chromatin with Haloacetaldehyde and Diethyl Pyrocarbonate.
B.H. Johnston, Hydroxylamine and Methoxylamine as Probes of DNA Structure.
M.A. Price and T.D. Tullius, Using Hydroxyl Radical to Probe DNA Structure.
C.S. Chow and J.K. Barton, Transition Metal Complexes as Probes of Nucleic Acids.
D.W. Ussery, R.W. Hoepfner, and R.R. Sinden, Probing DNA Structure With Psoralen in Vitro.
M.M. Becker and G. Grossmann, Photofootprinting DNA in Vitro.
H. Htun and B.H. Johnston, Mapping Adducts of DNA Structural Probes Using Transcription and Primer Extension Approaches.
F. Wohlrab, Enzyme Probes in Vitro.
Analysis of DNA Structure Inside Cells:
E. Palecek, Probing of DNA Structure in Cells with Osmium Tetroxide-2,2'*bBipyridine.
R.R. Sinden and D.W. Ussery, Analysis of DNA Structure in Vivo Using Psoralen Photobinding: Measurement of Supercoiling, Topological Domains, and DNA-Protein Interactions.
W. Zacharias, DNA Methylation in Vivo.
L.F. Liu and H.-Y. Wu, Topological Approaches to Studies of Protein-Mediated Looping of DNA in Vivo.
Interaction of DNA and Proteins:
D.G. Sanford and B.D. Stollar, Assay of Anti-DNA Antibodies.
P. Dr~adoge and A. Nordheim, Topoisomer Gel
Retardation: Protein Recognition of Torsional Stress-Induced DNA Conformations.
W.G. Turnell and A.A. Travers, Algorithms for Prediction of Histone Octamer Binding Sites.
T.M. Lohman and D.P. Mascotti, Thermodynamics of Ligand-Nucleic Acid Interactions.
T.M. Lohman and D.P. Mascotti, Nonspecific Ligand-DNA Equilibrium Binding Parameters Determined by Fluorescence Methods.
Author Index.
Subject Index.
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Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
"Incomparably useful."
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."
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