DNA Repair Protocols

DNA Repair Protocols : Mammalian Systems

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The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book's thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.show more

Product details

  • Hardback | 498 pages
  • 157.5 x 236.2 x 33mm | 952.56g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd Revised edition
  • biography
  • 1588295133
  • 9781588295132

Review quote

"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research "...must for any science library supporting a molecular biology program." - E-Streams "An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie "...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys. "This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newslettershow more

Back cover copy

In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair."show more

Table of contents

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating Malgorzata Z. Zdzienicka Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes Mathilde Frechet, Valerie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities William W. Au and Salama A. Salama Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation Shruti Nagar, James J. Corcoran, and William F. Morgan Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication George Iliakis, Ya Wang, and Hong Yan Wang Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage Xuan Huang and Zbigniew Darzynkiewicz Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication) Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi In Vitro Rejoining of Double-Strand Breaks in Genomic DNA George Iliakis and Nge Cheong Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan Plasmid-Based Assays for DNA End-Joining In Vitro George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants Rodney S. Nairn and Gerald M. Adair Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr Quantitative PCR-Based Measurement of Nuclear and MitochondrialDNA Damage and Repair in Mammalian Cells Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR Gerd P. Pfeifer Immunochemical Detection of UV-Induced DNA Damage and Repair Marcus S. Cooke and Alistair Robson A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts Shirley McCready Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay David L. Mitchell DNA Damage Quantitation by Alkaline Gel Electrophoresis Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair Gunter Speit and Andreas Hartmann Fast Micromethod DNA Single-Strand-Break Assay Heinz C. Schroder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Muller 32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA Vaughn Smider, Byung Joon Hwang, and Gilbert Chu Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision Huixian Wang and John B. Hays Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites Walter A. Deutsch and Vijay Hegde Base Excision Repair in Mammalian Cells Yoshihiro Matsumoto In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti Biochemical Assays for the Characterization of DNA Helicases Robert M. Brosh, Jr. and Sudha Sharma Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cellshow more