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DNA Barcodes : Methods and Protocols

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Description

A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.show more

Product details

  • Hardback | 470 pages
  • 180.34 x 251.46 x 33.02mm | 1,043.26g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2012
  • 51 Tables, black and white; XV, 470 p. 72 illus., 54 illus. in color.
  • 1617795909
  • 9781617795909
  • 1,312,580

Back cover copy

A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.show more

Table of contents

I. Introduction 1. Introduction (W. John Kress & David L. Erickson, Smithsonian, USA) II. DNA Barcodes for the Tree of Life2. Introduction to Animal DNA Barcoding ProtocolsLee A. Weigt, Amy Driskell, Andrea Ormos, Chris Meyer, and Allen Collins3. DNA Barcodes for the Tree of Life - A. Animals - InsectsJohn James Wilson and Scott E. Miller4. Barcoding Methods for InvertebratesNathaniel Evans & Gustav Paulay5. DNA Barcoding of Amphibians and ReptilesMiguel Vences, Zoltan T. Nagy, Gontran Sonet, Erik Verheyen6. DNA Barcoding FishesLee A. Weigt, Amy Driskell, Andrea Ormos7. DNA Barcoding Birds: from Field Collection to Data AnalysisDario A. Lijtmaer, Kevin C. R. Kerr, Mark Y. Stoeckle and Pablo L.Tubaro8. DNA Barcoding in MammalsNatalia V. Ivanova, Elizabeth L. Clare, Alex V. Borisenko 9. Methods for DNA Barcoding of Fungi Ursula Eberhardt10. Methods for DNA Barcoding Photosynthetic Protists Emphasizing the Macroalgae and DiatomsGary W. Saunders and Daniel C. McDevit11. DNA Barcoding Methods for Land PlantsAron J. Fazekas, Maria L. Kuzmina, Steven G. Newmaster, and Peter M. HollingsworthIII. Generating DNA Barcode Data12. Field Information Management Systems (FIMS) for DNA Barcoding Deck, John; Gross, Joyce; Stones-Havas, Steven; Davies, Neil1; Shapley, Rebecca & Meyer, Christopher13. Laboratory Information Management Systems (LIMS) for DNA Barcoding Meaghan Parker; Steven Stones-Havas, Craig Starger and Chris Meyer14. DNA Extraction, Preservation and Amplification Thomas Knebelsberger and Isabella Stoger15. DNA Mini-barcodesMehrdad Hajibabaei and Charly McKenna16. Next Generation Sequencing: Multiplexing Multiple Barcode Amplicons into Single 454 RunRyuji Machida and Nancy Knowlton IV. Applications of DNA Barcode Data 17. The Practical Evaluation of Barcode EfficacyJohn L. Spougeand Leonardo Marino-Ramirez 18. Plant DNA Barcodes, Taxonomic Management and Species Discovery in Tropical ForestsChristopher W. Dick and Campbell O. Webb19. Construction and Analysis of Phylogenetic Trees using DNA Barcode DataDavid L. Erickson and Amy C. Driskell20. Phylogenetic Analyses of Ecological Communities Using Barcode DataNathan G. SwensonV. Case Studies on DNA Barcodes21. DNA Barcodes: FISH-BOL, a Case StudyRobert D. Ward22. Generating Plant DNA Barcodes for Trees in Long-term Forest Dynamics PlotsW. John Kress, Ida C. Lopez, David L. Erickson23. Future DirectionsDavid L. Erickson and W. John Kressshow more

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