Cytokines and Colony Stimulating Factors

Cytokines and Colony Stimulating Factors : Methods and Protocols

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The immune system is a complex network in which different cell types and soluble factors interact to efficiently eliminate various kinds of microorganisms as well as aberrant cell clones. The roots of immunologic investigations reach far into the past. In 430 BC, Thucydides reported that survivors of the plague did not present a second time with similar symptoms. The first report of a successful immu- therapy was made by Edward Jenner in 1798 who found a protective effect of cowpox vaccination against human pox. Since then, much knowledge has been accumulated; today, investigations of the molecular mechanisms of immune regulation are of central research interest. The novel insights into gene polymorphisms and gene regulation gathered from this work has improved our knowledge of individual immune reactions and risk factors in overcoming infections. Strategies to use the immune system for cancer treatment have been propelled by the discovery of divergent immunoregulatory cytokines and the introduction of new gene therapy strategies to modify immune responses. Recently, the discovery of various dendritic cells has focused attention on these cell types as central elements of the immune response and to the possibility of dendritic cell expansion, maturation, and consecutive stimulation with immuno- active tumor-specific peptides. Similarly, methods for ex vivo expansion of various stem cell-derived cell types have led to an improved therapeutic management of various benign and malignant diseases.show more

Product details

  • Hardback | 478 pages
  • 156 x 234 x 28mm | 780.19g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • biography
  • 1588290352
  • 9781588290359

Back cover copy

Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques. Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy.show more

Table of contents

Part I. Introduction Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors Volker Wahn Part II. Detection Assays for Cytokines and Growth Factors Intracellular Detection of T-Cell Cytokines: Differentiation of TH1 and TH2 Cells Ursula Banning and Dieter Korholz Analysis of Cytokine Profiles in Human Skin Jean Krutmann, Renz Mang, and Susanne Grether-Beck Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry Christian Schultz Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells Kerstin Willmann Evaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow Cytometry Bodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk, and Florian Kern Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms Thomas Lehrnbecher and Stephen J. Chanock Apoptosis Induction by TRAIL Angelika Eggert, Hauke Sieverts, and Henning Walczak Angiogenic Cytokines: Quantitative and Functional Analysis Angelika Eggert and John H. Maris Part III. Protocols on Gene Therapy Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy: An Overview Christof M. Kramm, Tim Niehues, and Nikolai G. Rainov Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins Ciriaco A. Piccirillo and Gerald J. Prud'homme Interferon-b Gene Therapy for the Treatment of Arthritis Yuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, and David Gould Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination Tohru Sakai and Kunisuke Himeno Purification of the Eukaryotic Heat-Shock Proteins Hsp70 and gp96 Arne von Bonin, Solveig H.More, and Minka Breloer Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-a Moiety Jim Xiang and John R. Gordon Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-a Gene Transfer Jim Xiang and Josh Wu Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes Timothy M. Clay and Michael I. Nishimura Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus Glyn D. Palmer, Elvire Gouze, Jean-Noel Gouze, Oliver B. Betz, Christopher H. Evans, and Steven C. Ghivizzani Gene Therapy of X-Linked Severe Combined Immunodeficiency Salima Hacein-Bey-Abina, Genevieve de Saint Basile, and Marina Cavazzana-Calvo Gene Transfer for Generation of Tumor and Leukemia Vaccines Dagmar Dilloo and Andree Zibert Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors Roberto Furlan, Stefano Pluchino, Peggy C. Marconi, and Gianvito Martino Part IV. Ex Vivo Cell Expansion In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease Thomas Vraetz, Peter D. Emanuel, and Charlotte M. Niemeyer In Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitors Cells Bruno Canque, Michele Rosenzwajg, and Jean Claude Gluckman Generation of Transgenic T Cells from Human CD34+ Cord Blood Cells Bruno Verhasselt, Evelien Naessens, and Veronique Stove Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers Martin Bornhauser Ex Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static Culture Sergio Querol Ex Vivo Expansion of Hematopoietic Stem Cells Beatriz Albella, Joseshow more