Culture Media, Solutions, and Systems in Human ART
This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
- Electronic book text
- 05 Apr 2014
- CAMBRIDGE UNIVERSITY PRESS
- Cambridge University Press (Virtual Publishing)
- Cambridge, United Kingdom
- 56 b/w illus. 6 colour illus. 19 tables
Table of contents
Foreword Alan Trounson; Part I. Culture Media and Solutions: 1. Overview Patrick Quinn; 2. Media and embryo interactions Patrick Quinn; 3. Female tract environment and its relationship to ART media composition Henry J. Leese and Sarah Louise Whitear; 4. Buffering systems in IVF Natalie A. Clark and Jason E. Swain; 5. Essential features in media development for spermatozoa, oocytes and embryos David Mortimer; 6. Macromolecular supplementation of embryo culture media Thomas B. Pool; 7. Monozygotic twinning and assisted reproduction. Laboratory or clinical? Marius Meintjes; 8. Amino acids and ammonium Deirdre Zander-Fox and Michelle Lane; 9. Growth factors and cytokines in embryo development Sarah A. Robertson and Jeremy G. Thompson; 10. Osmolality Jay M. Baltz; 11. pH control in the embryo culture environment Joe Conaghan; 12. In vitro maturation of immature human oocytes Ri-Cheng Chian, Shan-Jun Dai and Yao Wang; 13. Oocyte and embryo cryopreservation media Diana Patricia Bernal, Ching-Chien Chang and Zsolt Peter Nagy; 14. International regulation of ART media Theresa Jeary; Part II. Culture Systems: 15. Culture of embryos in dynamic fluid environments Andre Monteiro da Rocha and Gary Daniel Smith; 16. Time-lapse imaging of embryo development: using morphokinetic analysis to select viable embryos Markus H. M. Montag, Kamilla S. Pedersen and Niels B. Ramsing; 17. Optimizing culture conditions Kathleen A. Miller; 18. Low cost IVF Luca Gianaroli, M. Cristina Magli and Anna P. Ferraretti; 19. Incubators old and new Eduardo Kelly and Takeo Cho; 20. Incubator management William R. Boone and H. Lee Higdon, III; 21. Summary and conclusions Patrick Quinn; Index.