Cryopreservation and Freeze-Drying Protocols

Cryopreservation and Freeze-Drying Protocols

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Description

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.


The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.
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Product details

  • Hardback | 347 pages
  • 154.9 x 231.1 x 25.4mm | 589.67g
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2007
  • 15 Tables, black and white; XI, 347 p.
  • 1588293777
  • 9781588293770

Table of contents

Contents

Preface

Contributors

Chapter 1
Long-term ex situ conservation of Biological Resources and the role of Biological Resource Centres
Glyn N. Stacey and John G. Day

Chapter 2
The Process of Freeze-drying
Gerald Adams

Chapter 3
Principles of Cryopreservation
David E. Pegg

Chapter 4
Lyophilization of Proteins
Paul Matejschuk

Chapter 5
Vacuum-drying and Cryopreservation of Prokaryotes
Brian J. Tindall

Chapter 6
Freeze-drying of Yeast Cultures
Chris Bond

Chapter 7
Cryopreservation of Yeast Cultures
Chris Bond

Chapter 8
Freeze-drying Fungi using a Shelf-freeze-drier
C. Shu-hui Tan, Cor W. van Ingen and Joost A. Stalpers

Chapter 9
Cryopreservation and Freeze-drying of Fungi employing Centrifugal and Shelf Freeze-drying
Matthew J. Ryan and David Smith

Chapter 10
Cryopreservation of Microalgae and Cyanobacteria
John G. Day

Chapter 11
Cryopreservation of Plant Cell Suspensions
Brian W. W. Grout

Chapter 12
Cryopreservation of Shoot-tips and Meristems
Erica E. Benson, Keith Harding and Jason W. Johnston

Chapter 13
Cryopreservation of Desiccation Tolerant Seeds
Hugh W. Pritchard

Chapter 14
Cryopreservation of Fish Sperm
Eugeny Kopeika, Julia Kopeika and Tiantian Zhang

Chapter 15
Cryopreservation of Avian Spermatozoa
Graham Wishart

Chapter 16
Cryopreservation of Animal and Human Cell Lines
Christopher B. Morris

Chapter 17
Cryopreservation of Haematopoietic Stem/Progenitor Cells for Therapeutic Use
Suzanne M. Watt, Eric Austin and Sue Armitage

Chapter 18
Cryopreservation of Human Embryonic Stem Cell Lines
Charles J. Hunt and Paula M. Timmons

Chapter 19
Cryopreservation of Primary Animal Cell Cultures
Glyn N. Stacey and Stuart Dowall

Chapter 20
Cryopreservation of Red Blood Cells and Platelets
Andreas Sputtek

Chapter 21
Cryopreservation of Mammalian Semen
Mark R. Curry

Chapter 22
Cryopreservation of Mammalian Oocytes
Sharon J. Paynter and Barry J. Fuller

Chapter 23
Cryopreservation of Mammalian Embryos
Barry J. Fuller and Sharon J. Paynter

Glossary
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Review Text

From the reviews of the second edition:

"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research

"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume ... ." (Peter Green, Molecular Biotechnology, December, 2007)
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Review quote

From the reviews of the second edition:


"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research


"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume ... ." (Peter Green, Molecular Biotechnology, December, 2007)
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