Cryo-EM: Cryo-EM Part A: Sample Preparation and Data Collection : Part A Volume 481

Cryo-EM: Cryo-EM Part A: Sample Preparation and Data Collection : Part A Volume 481

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Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
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Product details

  • Hardback | 464 pages
  • 158.75 x 228.6 x 25.4mm | 861.82g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 481st edition
  • 0123749069
  • 9780123749062

Table of contents

Historical perspective

3D reconstruction from electron micrographs: a personal account of its development

David DeRosier

Preparation of 2-D crystals
Henning Stahlberg

Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie

Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen

A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski

GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark

Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark

Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang

Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky

Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld

How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun

Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng

Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat

Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jurgen M. Plitzkod, Cindi L. Schwartz

Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama

Radiation damage in electron cryomicroscopy

John L. Rubinstein, Lindsay A. Baker
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