Constitutive Activity in Receptors and Other Proteins, Part B: Volume 485

Constitutive Activity in Receptors and Other Proteins, Part B: Volume 485

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This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.
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Product details

  • Hardback | 708 pages
  • 157.48 x 231.14 x 33.02mm | 1,133.98g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0123812968
  • 9780123812964

Table of contents

Identification and characterization of Steroidogenic Factor-1 inverse agonists.
Mabrouka Doghman, Franck Madoux, Peter Hodder, and Enzo Lalli

Assessment of inverse agonism for the angiotensin II type 1 receptor
Hiroshi Akazawa, Noritaka Yasuda, Shin-ichiro Miura, Issei Komuro

Measurement of inverse agonism in BARs
Carlos Alberto Taira

Inverse agonism of antidepressants: in vitro and in vivo studies
Joel Bockaert

Differential inverse agonism at the human muscarinic M3 receptor
Paola Casarosa

Ghrelin Receptor: High Constitutive Activity and Methods for Developing Inverse Agonists.
Constance Chollet

Constitutive activity and inverse agonism at the α1a and α1b adrenergic receptor subtypes.
Susanna. Cotecchia

Measurement of inverse agonism of the cannabinoid receptor
Tung Fong

Constitutively Active Thyrotropin (TSH) and Thyrotropin-Releasing Hormone (TRH) Receptors and Their Inverse Agonists
Marvin C. Gershengorn

Inverse retinoid agonists and neutral antagonists
Hinrich Gronemeyer

γ-Aminobutyric Acid Type A (GABAA) Receptor Subtype Inverse Agonists as Therapeutic Agents in Cognition
Gabriella Guerrini and Ciciani Giovanna

Assays for inverse agonists in the visual system.
Masahiro Kono

Receptor-driven identification of novel human A3 adenosine receptor antagonists as potential therapeutic agents
Silvia Paoletta, Stephanie Federico, Giampiero Spalluto and Stefano Moro

Inverse agonists of steroidogenic factor SF-1
Fabrice Piu and Andria L. Del Tredici

Methods to Measure G Protein Coupled Receptor Activity for the Identification of Inverse Agonists
Gabriel Barreda-Gomez, M.Teresa Giralt and Rafael Rodriguez-Puertas

Section II-- Novel Strategies and Techniques for Constitutive Activity and Inverse Agonism

Use of Pharmacoperones to Reveal GPCR Structural Components Associated with Receptor Activation and Trafficking
Jo Ann Janovick and P. Michael Conn

Application of large-scale transiently transfected cells to functional assays of ion channels and other proteins.
Jun Chen, Sujatha Gopalakrishnan, Marc R Lake, and Bruce R Bianchi

Quantification of RNA Editing of the Serotonin 2C Receptor (5-HT2CR) Ex Vivo
Maria Fe Lanfranco, Noelle C. Anastasio, Patricia K. Seitz, and Kathryn A. Cunningham

Strategies for isolating constitutively-active and dominant-negative pheromone receptor mutants in yeast.
Mercedes Dosil and James B. Konopka

Development of a GPR23 Cell-based ss-Lactamase Reporter Assay
Paul H. Lee and Bonnie J. Hanson

Computational Modeling of Constitutively Active Mutants of GPCRs: C5a Receptor.
Gregory V. Nikiforovich and Thomas J. Baranski

TSH receptor monoclonal antibodies with agonist, antagonist and inverse agonist activities
Jane Sanders, Ricardo Nunez Miguel, Jadwiga Furmaniak, Bernard Rees Smith

Current standards, variations and pitfalls for the determination of constitutive TSHR activity in vitro
Sandra Mueller, Holger Jaeschke, Ralf Paschke

Towards the Rational Design of Constitutively Active KCa3.1 Mutant Channels
Line Garneau, Helene Klein, Lucie Parent, Remy Sauve

Fusion proteins as model systems for the analysis of constitutive GPCR activity
Erich H. Schneider, Roland Seifert

Screening for Novel Constitutively Active CXCR2 Mutants and Their Cellular Effects
Giljun Park, Tom Masi, Chang K. Choi, Heejung Kim, Jeffrey M. Becker, and Tim E. Sparer

A method for parallel solid-phase synthesis of iodinated analogues of the cannabinoid receptor type I (CB1) inverse agonist rimonabant
Alan C. Spivey and Chih-Chung Tseng

Coexpression systems as models for the analysis of constitutive GPCR activity
Roland Seifert and Erich H. Schneider

Modelling and simulation of inverse agonism dynamics
Lloyd J. Bridge

Design and Use of Constitutively Active STAT5 Constructs
Michael A. Farrar

In vitro and in vivo assays of protein kinase CK2 activity

Renaud Prudent, Celine F. Sautel, Virginie Moucadel, Beatrice Laudet, Odilhe Filhol, and Claude Cochet
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