Confocal Microscopy

Confocal Microscopy : Methods and Protocols

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Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.show more

Product details

  • Hardback | 381 pages
  • 180.34 x 254 x 12.7mm | 816.46g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2014
  • 18 Tables, black and white; 51 Illustrations, color; 79 Illustrations, black and white; XI, 381 p. 130 illus., 51 illus. in color.
  • 1588293513
  • 9781588293510
  • 2,386,425

Back cover copy

Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.show more

Table of contents

1. Reflecting on Confocal Microscopy: A Personal Perspective John White 2. Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques Stephen W. Paddock and Kevin W. Eliceiri 3. Confocal Microscopy on the Internet Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko, Anna P. Ozarowska, Adam M. Rainey and Michael W. Davidson 4. Using Photoshop with Images Created by a Confocal System Jerry Sedgewick 5. Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy Timo Zimmermann, Joanne Marrison, Karen Hogg, and Peter O'Toole 6. Low Magnification Confocal Microscopy of Tumor Angiogenesis George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster, Yiwen Li, and Rong Wen 7. Confocal Imaging of Butterfly Tissue Craig R. Brunetti 8. Confocal Microscopy of Cardiac Myocytes Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, and Louis Terracio 9. Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction Ian P. Coyle 10. Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens Angela V. Klaus, Valerie Schawaroch, and Kevin J. Frischmann 11. Vital Imaging of Multicellular Spheroids Paulo J. Oliveira, Ed Perkins, and Jon Holy 12. Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos Barbara Fasulo and William Sullivan 13. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells Kevin F. O'Connell and Andy Golden 14. Measurement in the Confocal Microscope Guy Cox 15. A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung Robert L. Conhaim, Dennis M. Heisey, and Greg E. Leverson 16. Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini, and John F. Bertram 17. Evaluating Confocal Microscopy System Performance Robert M. Zuckershow more