Cilia, Part B: Volume 525

Cilia, Part B: Volume 525

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
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Product details

  • Hardback | 498 pages
  • 157.48 x 231.14 x 25.4mm | 816.46g
  • Academic Press Inc
  • San Diego, United States
  • English
  • New
  • 0123979447
  • 9780123979445

Table of contents

Methods for studying ciliary polarity in Xenopus

Analysis of signaling pathways in mammalian spermatozoa
Analysis of tubulin modification in zebrafish cilia
Analysis of microtubule end-binding proteins in cilia
Small molecule screens using Chlamydomonas flagella
Biochemical and Physiological analysis of axonemal dyneins
Analysis of ciliary assembly and function in Planaria
Reconstitution of flagellar sliding
Comparative genomic analysis of ciliary genes
Analysis of ciliogenesis in Tetrahymena
Measuring the regulation of dynein activity during flagellar motility
Centrosome isolation and proteomics
Probing ciliogenesis using micropatterned substrates
3D structural analysis of flagella/cilia by electron cryo-tomography
Visualizing IFT in mammalian cilia
Ciliogenesis in multiciliated epithelial cells in culture
Methods for analysis of PKD-containing urinary exosomes
Analysis of Interactions between IFT Proteins
Electron microscopy of Intraflagellar Transport
Using the SNAP tag to measure ciliary trafficking
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About Wallace F. Marshall

Wallace Marshall is an electrical engineer by training, who became interested in biology out of a desire to understand how cells solve engineering problems, such as determining the size of organelles. He received his Ph.D. at UCSF with John Sedat, where he studied the diffusional of motion of interphase chromatin using live cell imaging and computational image analysis. He then trained as a postdoc with Joel Rosenbaum at Yale, where he began studying the mechanisms regulating the length of cilia and flagella. He is now Profess of Biochemistry at UCSF, where he lab continues to study the assembly and length regulation of cilia and flagella, as well as the mechanisms that regulate the size of other organelles. His work takes advantage of an integrated combination of methods including genetics, microscopy, and computational modeling, as well as a wide variety of model organisms including Chlamydomonas reinhardtii, Stentor coeruleus, yeast, flatworms, and mammalian cells.
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