Chromatin Protocols: Preliminary Entry 2015
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Chromatin Protocols: Preliminary Entry 2015

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Description

Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology(TM) series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.show more

Product details

  • Hardback | 416 pages
  • 196 x 262 x 26mm | 997.9g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2009
  • biography
  • 1588298736
  • 9781588298737

Back cover copy

Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology. "show more

Review quote

From the reviews of the second edition: "This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. ... In summary, this is a very useful text, with lots of strengths ... ." (Ray Waters, Human Genetics, Vol. 126, 2009)show more

Table of contents

1. Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos Edgar Bonte and Peter B. Becker 2. Analysis of Reconstituted Chromatin Using a Solid-Phase Approach Raphael Sandaltzopoulos and Peter B. Becker 3. In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins Sherry L. Winter, Philip Wong, and Mark G. Alexandrow 4. DNA Methyltransferase Probing of Chromatin Structure within Populations and on Single Molecules Carolina Pardo, Scott A. Hoose, Santhi Pondugula, and Michael P. Kladde 5. Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues Takashi Furusawa and Michael Bustin 6. Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin Eisuke Gotoh 7. Analysis of DNA Topology in Yeast Chromatin Randall H. Morse 8. Preparation and Analysis of Uniquely Positioned Mononucleosomes Daria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, and Vasily M. Studitsky 9. Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy Michael J. Kruhlak, Arkady Celeste, and Andre Nussenzweig 10. Methods to Study Transcription-Coupled Repair in Chromatin Helene Gaillard, Ralf Erik Wellinger, and Andres Aguilera 11. Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs) Toshiki Tanaka, Dorota Halicka, Frank Traganos, and Zbigniew Darzynkiewicz 12. Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay Lori A.Hazlehurst 13. Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes Melanie Carless 14. In Vitro Replication Assay with Mammalian Cell Extracts Wasia Rizwani and Srikumar P. Chellappan 15. Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain Reaction Melanie Carless 16. Biochemical Analysis of Arginine Methylation in Transcription Marc Tini, Hina Naeem, and Joseph Torchia 17. In Vitro Histone Demethylase Assays Kenji Kokura and Jia Fang 18. Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation Shrikanth S. Gadad, Jayasha Shandilya, Venkatesh Swaminathan, and Tapas K. Kundu 19. Histone Deacetylase Activity Assay Zhigang Yuan, Natalie Rezai-Zadeh, Xiaohong Zhang, and Edward Seto 20. In vitro and in vivo Assays for Studying Histone Ubiquitination and Deubiquitination Ling Zhai, Heui-Yun Joo, and Hengbin Wang 21. Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining Jaya Padmanabhan 22. Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications in vivo Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan 23. ChIP on Chip Assays: Genomewide Analysis of Transcription Factor Binding and Histone Modifications Smitha Pillai and Srikumar P. Chellappan 24. ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays Sean J. Yoder and Steven A. Enkemann 25. Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length Partha P. Banerjee and Shankar Jagadeesh 26. Detecting ATM Dependent Chromatin Modification in DNA Damage ashow more

Review Text

From the reviews of the second edition: "This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. ... In summary, this is a very useful text, with lots of strengths ... ." (Ray Waters, Human Genetics, Vol. 126, 2009)show more