Cellulases: Volume 510

Cellulases: Volume 510

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Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."
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Product details

  • Hardback | 552 pages
  • 152.4 x 231.14 x 25.4mm | 898.11g
  • Academic Press Inc
  • San Diego, United States
  • English
  • New
  • 0124159311
  • 9780124159310
  • 1,771,358

Table of contents

Measurement of endo-1,4-b-glucanase
Biomass Conversion determined via Fluorescent Cellulose Decay assay
The analysis of saccharification in biomass using an automated high-throughput method
Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
Measuring processivity
Distinguishing xyloglucanase activity in endo-b (1 (R) 4) glucanases
Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
Quantitative approaches to the analysis of carbohydrate-binding module function
In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
Approaches for improving thermostability characteristics in cellulases
Thermophilic glycosynthases for oligosaccharides synthesis
Engineering cellulase activity into Clostridium acetobutylicum
Transformation of Clostridium thermocellum by electroporation
Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
Methods for the isolation of cellulose-degrading microorganisms
Metagenomic approaches to the discovery of cellulases
Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
High-throughput screening of cohesin mutant libraries on cellulose microarrays
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