Cell Cycle Control

Cell Cycle Control : Mechanisms and Protocols

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The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that "all cells come from cells." In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases.
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Product details

  • Hardback | 404 pages
  • 157.5 x 236.2 x 25.4mm | 839.16g
  • Totowa, NJ, United States
  • English
  • 2005 ed.
  • XII, 404 p.
  • 1588291448
  • 9781588291448

Back cover copy

Our understanding of the cell cycle and its regulation has significantly increased with recent discoveries of links to DNA repair, growth, cellular metabolism, development, cell death, proliferative disease, cardiovascular diseases, and cancer. In Cell Cycle Control: Mechanisms and Protocols, internationally recognized researchers not only provide overviews of cell cycle regulatory mechanisms, but also set forth their best techniques for studying the eukaryotic cell cycle and its key regulatory molecules in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators-and those more recently defined-in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Cell Cycle Control: Mechanisms and Protocols offers all cell researchers an indispensable collection of critical reviews and techniques essential to investigate how normal cells divide and how this is altered in disease.
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Table of contents

Part I. Overviews of Cell Cycle Control in Different Organisms

Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts
Tim Humphrey and Amanda Pearce

The Plant Cell Cycle: An Overview
John H. Doonan

The C. elegans Cell Cycle: Overview of Molecules and Mechanisms
Sander van den Heuvel

Developmental Control of Growth and Cell Cycle Progression in Drosophila
Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio

The Xenopus Cell Cycle: An Overview
Anna Philpott and P. Renee Yew

The Mammalian Cell Cycle: An Overvew
Jane V. Harper and Gavin Brooks

Part II. Cell Cycle Study Methods and Techniques

Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
Jane V. Harper

Mapping Origins of DNA Replication in Eukaryotes
Susan A. Gerbi

In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
Stephen E. Kearsey, Lydia Brimage, Mandana Namdar, Emma Ralph, and Xiaowen Yang

Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs
Peter D. Adams

The Cell Cycle and Virus Infection
Stevan R. Emmett, Brian Dove, Laura Mahoney, Torsten Wurm, and Julian A. Hiscox

Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies
Julie Welburn and Jane Endicott

Part III. Detection and Measurement of Cell Cycle Molecules

E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
Ludger Hauck and Rudiger von Harsdorf

Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors
Katrina A. Bicknell

Measurement of Geminin Activity in Xenopus Egg Extracts
Thomas J. McGarry

CDK-ActivatingKinases: Detection and Activity Measurements
Stephane Larochelle and Robert P. Fisher

Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
Gavin Brooks

Measurement of Wee Kinase Activity
Paul R. Mueller and Walter F. Leise III

CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements
Sorab N. Dalal and Melanie Volkening

Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
Carmela Palermo and Nancy C. Walworth

Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown
Marcel A. T. M. van Vugt and Rene H. Medema

The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor

Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs
Mark L. McCleland and P. Todd Stukenberg

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