Cell Culture Engineering VI

Cell Culture Engineering VI

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The latest edition in this continuing series includes the newest advances in the rapidly evolving field of animal cell culture, genetic manipulations for heterologous gene expression, cell line enhancements, improved bioreactor designs and separations, gene therapy manufacturing, tissue engineering, anti-apoptosis strategies and cell cycle research. The contents include new research articles as well as critical reviews on emerging topics such as viral and viral-like agent contamination of animal cell culture components. These papers were carefully selected from contributions by leading academic and industrial experts in the biotechnology community at the recent Cell Culture Engineering VI Meeting in San Diego, USA, 1998. However, the book is not merely a proceedings.
Audience: Biochemical engineers, cell biologists, biochemists, molecular biologists, immunologists and other disciplines related to cell culture engineering, working in the academic environment and the biotechnology or pharmaceutical industry.
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Product details

  • Hardback | 235 pages
  • 195.6 x 261.6 x 17.8mm | 703.08g
  • Dordrecht, Netherlands
  • English
  • New edition
  • Reprinted from CYTOTECHNOLOGY, 28:1-3
  • VI, 235 p.
  • 079235575X
  • 9780792355755

Table of contents

Cell therapy in kidney failure; H.D. Hunes, et al. Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE); T.L. Aldrich, et al. Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination; A. Hawerkamp, et al. Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line; B. Rasmussen, et al. Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them; S.J. Wessmann, R.L. Levings. An overview of viral and viral-like agents in cell culture systems; J.C. Petricciani. New adenovirus vectors for protein production and gene transfer; B. Massie, et al. Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue; F. Franek, et al. Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth-associated production of a foreign protein, beta-galactosidase; F.W.F. Lee, et al. A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy; G. Schoofs, et al. Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3; N-A. Sunstrom, et al. Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions; J. Varani, et al. Regulated multicistronic expression technology for mammalian metabolic engineering; M. Fussenegger, et al. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions; A. de Leon, et al. Historical reflections on cell culture engineering; A.S.Lubiniecki. Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation; J.H. Parham, et al. Population balance model of in vivo neutrophil formation following bone marrow rescue therapy; L.K. Nielsen, et al. Mammalian cell retention devides for stirred perfusion bioreactors; S.M. Woodside, et al. Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells; A. Perani, et al. Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions; S. Mercille, B. Massie. Effects of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures; E-J. Schlaeger, K. Lundstrom. Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production; V.M. deZengotita. pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons; M. Fussenegger, et al.
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