Cell Biology : A Laboratory Handbook
This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together 260 new and revised chapters. Each chapter is concisely written and beautifully illustrated, making this attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to assemble this much needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors.
- Spiral bound | 2400 pages
- 159.5 x 236.7 x 25.9mm | 855.95g
- 16 Dec 1997
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
- 2nd Revised edition
- colour illustrations, b&w illustrations, index
The terms bible and encyclopedic collection are often used of scientific works aspiring to present to the scientific community all the branches of a discipline. Often these terms are misleading! Here they are paradigmatic... could not be more accurate and user-friendly... The book is not only helpful for writing papers, but invaluable for bench work... a masterpiece among scientific publications. --EUROPEAN JOURNAL OF HISTOCHEMISTRY This compendium is a welcome addition to the cell biology laboratory, assembling a wide array of techniques of value to cell biologists? Contributions are by recognized experts in their fields, in some cases the originator of the technique or procedure. The four volumes are illustrated with numerous black-and-white photos of cells, instruments, and experimental set-ups, as well as black-and white drawings to assist the reader in understanding what to expect from the protocols. --Stephen A. Adam, Ph.D., Northwestern University Medical School, for DOODY'S PUBLISHING The second edition of this highly praised handbook brings together more than 240 new, revised, concisely written and beautifully illustrated chapters in an attractive four-volume set, providing and easy-to-use source of current and classic protocols, presented in a clear format. --JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (1999) This compendium is a welcome addition to the cell biology laboratory, assembling a wide array of techniques of value to cell biologists...Contributions are by recognized experts in their fields, in some cases the originator of the technique or procedure. The four volumes are illustrated with numerous black-and-white photos of cells, instruments, and experimental set-ups, as well as black-and white drawings to assist the reader in understanding what to expect from the protocols. --CHOICE @from:Praise for the First Edition The writing is uniformly clear and precise. The editor is to be praised for a superb handbook which should be essential for any biological or biochemical department. --J. Chayen in CELL BIOCHEMISTRY AND FUNCTION I look forward to the publication of the Handbook with great interest. There is clearly an enormous need for such a book, and I think the cell biology community--and my students--will be grateful. --Tim Mitchison, University of California, San Francisco The aim of Cell Biology: A Laboratory Handbook is to assemble in one place the most important methods in cell biology, which have withstood the test of time to become classics. This goal has been admirably achieved in a three-volume-set edited by Julio E. Celis. This Handbook is divided into 15 sections, starting with techniques for culturing and analyzing whole cells, progressing through sections on cell fractionation, microscopy, generation of antibodies, introduction of molecules into living cells, and ending up with methods for analysis of proteins. Chapters are short and to the point. Methods are described in sufficient detail to allow the techniques to be reproduced, and are provided as easy-to-follow recipes. Importantly, the pitfalls inherent in each technique are assembled at the end of each article. The layout is admirably clear, and the quality of the illustrations is superb. The compendium will undoubtedly prove invaluable to cell biologists and to molecular biologists who wish to study the role of macromolecules in cell function. Cell Biology: A Laboratory Handbook should become the bible of established cell biological methods. --Tony Hunter, Salk Institute, La Jolla, California The new Handbook is likely to become the standard laboratory manual in cell biology. It is an encyclopedic collection of methods used in cell biology laboratories and provides one stop shopping for much of the practical advice that the experimentalist needs to try new approaches to trouble shoot out ones... Short of having an expert at your side or a collection of smaller books on tissue culture, microscopy, and routine biochemical methods, this is probably the best way to introduce new methods into a cell biology laboratory? The important advantage here is that the whole range of topics is presented conveniently together in an easily accessible format. --Thomas D. Pollard, Johns Hopkins University, School of Medicine What a magnificent collection of books this Handbook constitutes, perfectly well adapted for the laboratory techniques and consequently for a daily use of all scientist working in life sciences. --CELLULAR AND MOLECULAR BIOLOGY In summary, Cell Biology: A Laboratory Handbook is the type of an essential manual that is easily accessible to potential students as well as scientists from both research laboratories and biotechnology companies. --Jiri Bartek, Dansk Center for Humna Genom Forskning The three volumes of Cell Biology: A Laboratory Handbook cover recipes for all possible techniques applicable to cell biological problems, and a few more. Now, all our experiments are bound to be a success--at least in theory. --TRENDS IN CELL BIOLOGY The illustrations throughout the manual are extremely clear and high in quality. The catalog numbers for products are a major help in applying the protocols and comments and pitfalls are welcome and realistic. The list of authors and contributors is extensive and specialists throughout the world have been consulted. --CELL BIOLOGY INTERNATIONAL
Table of contents
Volume 1: Cell and tissue culture and asssociated techniques; viruses. Volume 2: Organelles and cellular structures; assays; antibodies. Volume 3: Light microscopy and contrast generation; electron microscopy; intracellular measurements; cytogenetics and "in situ" hybridization; transgenic and gene knockouts. Volume 4: Transfer of macromolecules and small molecules; expression systems; differential gene expression; proteins. (part contents)
About Julio E. Celis
A native of Chile, Dr. Julio E. Celis received his bachelor s degree from the University of Chile in 1964, and then completed his Ph.D. in the United States at the University of Iowa. After his postdoctoral training at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England, he went on to become an assistant professor at the University of Chile. Dr. Celis was named an associate professor in 1975 at Aarhus University, Denmark, and in 1987 became Chairman of the University s Institute of Medical Biochemistry. He is also the Chairman of the Danish Centre for Human Genome Research and was recently elected Secretary General-elect of the Federation of European Biochemical Societies (FEBS) 1997. With nearly 180 publications to his credit, Dr. Celis specialized areas of research include molecular mechanisms of cancer, human 2D gel protein databases and their link to genome data, signal transduction, and the biology of human skin. Married, with three children and one cat (Max William), Dr. Celis currently resides in Denmark where he holds among his many titles the chair to the Symposium and Prize Committee of the Danish Cancer Society, as well as the acting Chairman of the Nordic Molecular Biology Association (NOMBA). Dr. Celis is currently the Vice President of the European Molecular Biology Laboratory (EMBL) Council and leader of the Danish Delegation to the OECD Megascience Forum subgroup on Bioinformatics. He is also the European Union Observer to the International Nucleotide sequence database collaboration and a member of the EMBO Course Committee.