Cell Biology Assays

Cell Biology Assays : Proteins

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Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a vareity of technologies and model systems.
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Product details

  • Paperback | 394 pages
  • 190.5 x 233.68 x 22.86mm | 816.46g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0123756928
  • 9780123756923

About Kreitzer Geri

A native of Chile, Dr. Julio E. Celis received his bachelor's degree from the University of Chile in 1964, and then completed his Ph.D. in the United States at the University of Iowa. After his postdoctoral training at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England, he went on to become an assistant professor at the University of Chile. Dr. Celis was named an associate professor in 1975 at Aarhus University, Denmark, and in 1987 became Chairman of the University's Institute of Medical Biochemistry. He is also the Chairman of the Danish Centre for Human Genome Research and was recently elected Secretary General-elect of the Federation of European Biochemical Societies (FEBS) 1997. With nearly 180 publications to his credit, Dr. Celis' specialized areas of research include molecular mechanisms of cancer, human 2D gel protein databases and their link to genome data, signal transduction, and the biology of human skin. Married, with three children and one cat (Max William), Dr. Celis currently resides in Denmark where he holds among his many titles the chair to the Symposium and Prize Committee of the Danish Cancer Society, as well as the acting Chairman of the Nordic Molecular Biology Association (NOMBA). Dr. Celis is currently the Vice President of the European Molecular Biology Laboratory (EMBL) Council and leader of the Danish Delegation to the OECD Megascience Forum subgroup on Bioinformatics. He is also the European Union Observer to the International Nucleotide sequence database collaboration and a member of the EMBO Course Committee.
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Table of contents

Protein Determination
Phosphopeptide Mapping: A Basic Protocol
Coupling of Fluorescent Tags to Proteins
Radioiodination of Proteins and Peptides
Free-Flow Electrophoresis
Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteome Analysis
Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis of Differential Protein Expression in Multiple Biological Samples
Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal Antibodies into Monoclonal Antibodies in Murine Blood Plasma
Image Analysis and Quantitation
Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity in Flat Samples
Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in vivo Isotope Labeling
Immunoprecipitation of Proteins under Nondenaturing Conditions
Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation Complexes
Affinity purification with Natural Immobilized Ligands
Analysis of Protein-Protein Interactions by Chemical Cross-Linking
Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
Biomolecular Interaction Analysis Mass Spectrometry
Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping Protein-Protein Interaction Sites
Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins
Modular Scale Yeast Two-Hybrid Screening
Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with Malachite Green
Chromatin Immunoprecipitation (ChIP)
Gel Mobility Shift Assay
DNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping Approach
Protein Degradation Methods: Chaperone-Mediated Autophagy
Methods in Protein Ubiquitination
Protein Identification and Sequencing by Mass Spectrometry
Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
In-Gel Digestion of Protein Spots for Mass Spectrometry
Peptide Sequencing by Tandem Mass Spectrometry
Direct Database Searing Using Tandem Mass Spectra of Peptides
Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectrometry and Sequence-Similarity Database Searching Tools
Identification of Protein Phosphorylation Sites by Mass Spectrometry
Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry
Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics
Site-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide Mixtures
Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry
Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation of Methionine-Containing Peptides from Complex Peptide Mixtures
Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
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