Cartilage and Osteoarthritis: Cartilage and Osteoarthritis Structure and in Vivo Analysis v. 2
19%
off

Cartilage and Osteoarthritis: Cartilage and Osteoarthritis Structure and in Vivo Analysis v. 2

Free delivery worldwide

Available. Dispatched from the UK in 4 business days
When will my order arrive?

Description

An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.
show more

Product details

  • Hardback | 346 pages
  • 154 x 234 x 22mm | 762.05g
  • Totowa, NJ, United States
  • English
  • 2004 ed.
  • XIV, 346 p.
  • 1588295052
  • 9781588295057

Back cover copy

Osteoarthritis (OA), the most common form of arthritis, imposes a significant burden of suffering on a growing population of the elderly. Even today, its poorly understood pathophysiology limits the discovery of molecular targets for pharmacological intervention and there are few effective medical treatments beyond pain control and surgery. In Cartilage and Osteoarthritis a distinguished panel of researchers, physicians, and opinion leaders in this challenging field describe their updated classical, but still evolving, techniques, as well as many emerging methods that promise to add significantly to our understanding of cartilage metabolism in health and disease. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for studies-at the tissue level-of turnover, structure, and functioning in normal and diseased cartilage by invasive and noninvasive means. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Volume 1: Cellular and Molecular Tools, describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells.
Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists alike with an indispensable collection of readily reproducible protocols for new experiments-from the cellular to the animal level-designed to more clearly describe the pathophysiology of cartilage, as well as to discover novel molecular targets for pharmacological intervention.
show more

Table of contents

Generation and Use of Transgenic Mice As Models of Osteoarthritis
Anna-Marja Saamanen and Eero Vuorio

Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide
Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick Garnero

Histological and Immunohistological Studies on Cartilage
James Melrose, Susan Smith, and Peter Ghosh

Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe
James Melrose and Susan Smith

Methods for Cartilage and Subchondral Bone Histomorphometry
Philippe Pastoureau and Agnes Chomel

Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritis
Martin Judex, Elena Neumann, Steffen Gay, and Ulf Muller-Ladner

Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopy
Stephan Soeder, Alexander Kuhlmann, and Thomas Aigner

Molecular and Biochemical Assays of Cartilage Components
Caroline D. Hoemann

Mechanical Characterization of Native and Tissue-Engineered Cartilage
Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. Sah

Noninvasive Study of Human Cartilage Structure by MRI
Felix Eckstein

High-Resolution MRI Techniques for Studies in Small-Animal Models
Olivier Beuf

High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography
Lydia Wachsmuth and Klaus Engelke

High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes
Amena Saied and Pascal Laugier

Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance
Jurgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Joern Kaufmann, and Klaus Arnold

Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseases
Jurgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus Arnold

Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods
Daniel Huster, Jurgen Schiller, and Klaus Arnold

Computerized Protocol for Anatomical and Functional Studies of Joints
Sandra Martelli and Stefano Zaffagnini

Index
show more

Review quote

"This is a high quality laboratory manual that provides detailed instructions for conducting experiments directed at studying the role of cartilage in osteoarthritis. Overall, this would be a worthwhile book to keep in the laboratory for anyone studying the molecular mechanisms of connective tissue diseases."-Doody's Health Sciences Book Review Journal
show more