Capillary Electrophoresis

Capillary Electrophoresis : Theory and Practice

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This text draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on: electroendo-osmosis, separation efficiency, and joule heating; detection methods; the five major models of CE, with their respective separation principles and advantages.
The CE analogs of: sieving-gel electrophoresis and isoelectric focusing; free solution CE, micellar electrokinetic capillary chromatography; entangled polymer matrix-based separation; the application of CE to peptide, protein, and small molecule analysis, supplemented with extensive "hands-on" illustrations.
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Product details

  • Hardback | 352 pages
  • 157.48 x 215.9 x 22.86mm | 680.39g
  • Academic Press Inc
  • San Diego, United States
  • English
  • references, index
  • 012304250X
  • 9780123042507

Table of contents

Factors affecting the performance of capillary electophoresis separations - joule heating, electroosmosis, and zone dispersion, P.D. Grossman; detection methods in capillary electrophoresis, T.M. Olefirowicz and A.G. Ewing; quantitative aspects of capillary electrophoresis analysis, S.E. Moring; modes of capillary - free-solution capillary electrophoresis, P.D. Grossman; capillary gel electrophoresis, R.S. Dubrow; fundamentals of micellar electrolinetic capillary chromatography, M.J. Sepaniak, et al; iso-electric focusing in capillaries, S. Hjerten; capillary electrophoresis in entangled polymer solutions, P.D. Grossman; applications of capillary electrophoresis - capillary electrophoresis separations of peptides - practical aspects and applications, J.C. Colburn; protein analysis by capillary electrophoresis, J.E. Wiktorowicz and J.C. Colburn; separation of small molecules by high-performance capillary electrophoresis, C.W. Demarest, et al; Appendix - trouble-shooting guide to capillary electrophoresis operations, J.C. Colburn and P.D. Grossman; chapter references.
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