C. elegans

C. elegans : Methods and Applications

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Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of "naive reductionism. " Red- tionist methods will continue to be an essential element of all biological research efforts, but "naive reductionism," the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2-5). The "systems" of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or "emergent" properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.
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Product details

  • Hardback | 292 pages
  • 157.5 x 231.1 x 25.4mm | 612.36g
  • Totowa, NJ, United States
  • English
  • 2006 ed.
  • 42 Illustrations, black and white; XII, 292 p. 42 illus.
  • 1588295974
  • 9781588295972

Table of contents

An Overview of C. elegans Biology
Kevin Strange

Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species
Avril Coghlan, Jason E. Stajich and Todd W. Harris

WormBase: Methods for Data Mining and Comparative Genomics
Todd W. Harris and Lincoln D. Stein

C. elegans Deletion Mutant Screening
Robert J. Barstead and Donald G. Moerman

Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes
Jean-Louis Bessereau

SNP Mapping
M. Wayne Davis and Marc Hammarlund

Creation of Transgenic Lines Using Microparticle Bombardment Methods
Vida Praitis

Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA
Lisa Timmons

Delivery Methods for RNAi in Caenorhabditis elegans
Lisa Timmons

Functional Genomic Approaches in C. elegans
Todd Lamitina

Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins
Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian

Fluorescent Reporter Methods
Harald Hutter

Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans
Michael M. Francis and Andres Villu Maricq

Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis
Michael A. Miller

Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry
Robby M. Weimer

Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants
Keith Nehrke

Automated Imaging of C. elegans Behavior
Christopher J. Cronin, Zhaoyang Feng and William R. Schafer

Intracellular Ca2+ Imaging in Caehorhabditis elegans
Rex A. Kerr and William R. Schafer

In Vitro Culture of C. elegans Somatic Cells
Kevin Strange and Rebecca Morrison

Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS Flow Sorting System
Rock Pulak et al.
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