Bulletin Volume 6-19

Bulletin Volume 6-19

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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1894 edition. Excerpt: ...made by drawing about 20 c. c. of blood from the heart with sterile pipettes and transferring it to sterile tubes, which 1 This method was recommended 1 y Dr. Hillings to lie very efficacious in the detection of tliu specific bacillus of this disease. were exposed as soon as possible to a temperature of from 30 to 37 C, remained clear. The failure to discover a specific or even constantly present organism in the diseased animals was paralleled by the few independent cultures made by Dr. Niles, who assisted in many of the autopsies. The fact that he made independent cultures is important, as he used culture media prepared in his laboratory, while mine was made in the laboratory of the Bureau. Although presumably a given bacillus would grow equally well in both media, the fact that media from two laboratories were employed in making cultures from the same animal shows that the failure to obtain the Billings or other organism was presumably not due to any peculiar or deleterious property which the media used by me might for any reason have possessed. In 1892 it was impossible to make rabbit inoculations with the fresh tissues. In 1893, however, a few rabbits were inoculated with the blood and several with the brain tissue from Nos. 11 and 12. All of these rabbits remained well. The difficulties attending the bacteriological investigation of this disease are obvious, and the use of delicate methods for the cultivation of amerobic bacteria, as well as those necessary for the study of organisms requiring a constant temperature, can not well be made in field examinations. In these investigations cultures were made in sufficiently large numbers and with the necessary care to warrant the conclusion that there is no species of bacteria which can be...
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Product details

  • Paperback | 416 pages
  • 189 x 246 x 22mm | 739g
  • Rarebooksclub.com
  • Miami Fl, United States
  • English
  • black & white illustrations
  • 1236586107
  • 9781236586100