Bone Marrow and Stem Cell Transplantation

Bone Marrow and Stem Cell Transplantation

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The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.show more

Product details

  • Paperback | 313 pages
  • 178 x 254 x 22mm | 625g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • Softcover reprint of the original 2nd ed. 2014
  • 26 Tables, black and white; 35 Illustrations, color; 7 Illustrations, black and white; X, 313 p. 42 illus., 35 illus. in color.
  • 1493944304
  • 9781493944309
  • 1,583,701

Back cover copy

The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.show more

Table of contents

1. Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis Sophie Kusy and Christopher H. Contag 2. Flow Cytometry for Hematopoietic CellsDaniela S. Krause, Michelle E. DeLelys, Frederic I. Preffer 3. Isolation and Characterization of Mesenchymal Stem CellsSedat Odabas, A. Eser Elcin, and Y. Murat Elcin 4. Methods for Functional Analysis of Stem CellsAurore Saudemont, Michelle Escobedo-Cousin and J Alejandro Madrigal 5. An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation Katy Latham, Ann-Margaret Little, J Alejandro Madrigal 6. HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex (TM) Technology Klara Dalva and Meral Beksac 7. Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing ApproachChristina E.M. Voorter, Fausto Palusci and Marcel G.J. Tilanus 8. Molecular Typing Methods for Minor Histocompatibility AntigensEric Spierings 9. Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell TransplantationJennifer Schellekens, Katia Gagne, and Steven GE Marsh 10. Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow CytometryRichard Charles Duggleby and J Alejandro Madrigal 11. Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid DisordersAhmet H. Elmaagacli 12. Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell DisordersPaolo Corradini and Cristiana Carniti 13. Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell TransplantationPinar Yurdakul and Sule Colakoglu 14. Post-transplant Monitoring of Chimerism by Lineage-specific Analysis Sandra Preuner and Thomas Lion 15. Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell TransplantationEva M. Weissinger, William Mullen, and Amaya Albalatshow more