Blood'Brain Barrier

Blood'Brain Barrier : Biology and Research Protocols

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Blood-brain barrier (BBB) breakdown leading to cerebral edema occurs in many brain diseases-such as trauma, stroke, inflammation, infection, and tumors-and is an important factor in the mortality arising from these con- tions. Despite the importance of the BBB in the pathogenesis of these diseases, the molecular mechanisms occurring at the BBB are not completely und- stood. In the last decade a number of molecules have been identified not only in endothelial cells, but also in astrocytes, pericytes, and the perivascular cells that interact with endothelium to maintain cerebral homeostasis. However, the precise cellular interactions at a molecular level in steady states and d- eases have still to be determined. The introduction of new research techniques during the last decade or so provide an opportunity to study the molecular mec- nisms occurring at the BBB in diseases. The Blood-Brain Barrier: Biology and Research Protocols provides the reader with details of selected morphologic, permeability, transport, in vitro, and molecular techniques for BBB studies, all written by experts in the field. Each part is preceded by a review that emphasizes the advantages and pitfalls of particular techniques, as well as offering much relevant current information. The techniques provided will be helpful to both beginners in BBB research and those more experienced investigators who wish to add a specific technique to those already available in their laboratories.
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Product details

  • Hardback | 550 pages
  • 183.9 x 259.6 x 31mm | 1,501.41g
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • XIV, 550 p.
  • 1588290735
  • 9781588290731
  • 2,616,109

Back cover copy

Despite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and clinician-scientists present state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium.
Comprehensive and state-of-the-art, The Blood-Brain Barrier: Biology and Research Protocols offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.
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Table of contents

Part I. Tissue Techniques

Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels
Sukriti Nag

Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry
Sukriti Nag

Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions
Hartwig Wolburg, Stefan Liebner, and Andrea Lippoldt

Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy
Albert S. Lossinsky and Richard R. Shivers

Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy
Britta Engelhardt, Peter Vajkoczy, and Melanie Laschinger

Part II. Blood-Brain Barrier Permeability Techniques

Pathophysiology of Blood-Brain Barrier Breakdown
Sukriti Nag

Pial Vessel Permeability to Tracers Using Cranial Windows
William G. Mayhan

Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
Sukriti Nag

Ultracytochemical Studies of the Compromised Blood-Brain Barrier
Sukriti Nag

Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier
Eain M. Cornford, Shigeyo Hyman, and Marcia E. Cornford

Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers
Kamlesh Asotra, Nagendra Ningaraj, and Keith L. Black

Part III. Blood-Brain Barrier Transport Techniques

A Review of Blood-Brain Barrier Transport Techniques
Quentin R. Smith

In Situ Brain Perfusion Technique
Quentin R. Smith and David D. Allen

Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain Barrier
Hiroyuki Kusuhara, Tetsuya Terasaki, and Yuichi Sugiyama

Transport Studies Using Membrane Vesicles
Darryl R. Peterson and Richard A. Hawkins

Drug Transport Studies Using Quantitative Microdialysis
Haiqing Dai and William F. Elmquist

Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids
Michelle A. Puchowicz, Kui Xu, and Joseph C. LaManna

Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin
Laurence Fenart and Romeo Cecchelli

Transport Across the Choroid Plexuses In Vivo and In Vitro
Nathalie Strazielle and Jane Elizabeth Preston

Part IV. In Vitro Techniques

An Overview of In Vitro Techniques for Blood-Brain Barrier Studies
Andreas Reichel, David J. Begley, and N. Joan Abbott

Isolation and Characterization of Human Brain Endothelial Cells
Katerina Dorovini-Zis, Rukmini Premeya, and Hanh Huynh

Human Brain Microvessel Endothelial Cell and Leukocyte Interactions
Jacqueline Shukaliak-Quandt, Donald Wong, and Katerina Dorovini-Zis

Development and Characterization of Immortalized Cerebral Endothelial Cell Lines
Pierre-Olivier Couraud, John Greenwood, Francoise Roux, and Pete Adamson

Isolation and Characterization of Retinal Endothelial Cells
David A. Antonetti and Ellen B. Wolpert

Isolation and Characterization of Cerebral Microvascular Pericytes
Paula Dore-Duffy

Part V. Molecular Techniques

Molecular Biology of the Blood-Brain Barrier
William M. Pardridge

Blood-Brain Barrier Genomics
Ruben J. Boado

Dynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray Analysis
Matteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, and Damir Janigro

Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells
Jamshid Tanha, Arumugam Muruganandam, and Danica Stanimirovic

mRNA Detection in Cerebral Vessels by Nonradioactive In Situ
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Review quote

"An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier."-Doody's Health Science Book Review Journal
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