The Blood-Brain Barrier
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The Blood-Brain Barrier : Biology and Research Protocols

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Blood-brain barrier (BBB) breakdown leading to cerebral edema occurs in many brain diseases-such as trauma, stroke, inflammation, infection, and tumors-and is an important factor in the mortality arising from these con- tions. Despite the importance of the BBB in the pathogenesis of these diseases, the molecular mechanisms occurring at the BBB are not completely und- stood. In the last decade a number of molecules have been identified not only in endothelial cells, but also in astrocytes, pericytes, and the perivascular cells that interact with endothelium to maintain cerebral homeostasis. However, the precise cellular interactions at a molecular level in steady states and d- eases have still to be determined. The introduction of new research techniques during the last decade or so provide an opportunity to study the molecular mec- nisms occurring at the BBB in diseases. The Blood-Brain Barrier: Biology and Research Protocols provides the reader with details of selected morphologic, permeability, transport, in vitro, and molecular techniques for BBB studies, all written by experts in the field. Each part is preceded by a review that emphasizes the advantages and pitfalls of particular techniques, as well as offering much relevant current information. The techniques provided will be helpful to both beginners in BBB research and those more experienced investigators who wish to add a specific technique to those already available in their laboratories.show more

Product details

  • Hardback | 550 pages
  • 183.9 x 259.6 x 31mm | 1,501.41g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • biography
  • 1588290735
  • 9781588290731
  • 2,415,487

Back cover copy

Despite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and clinician-scientists present state-of-the-art morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood-brain barrier in health and disease. Described in step-by-step detail, each proven method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technique. At the cutting edge are techniques to explore the genomics and proteomics of brain endothelium. Comprehensive and state-of-the-art, The Blood-Brain Barrier: Biology and Research Protocols offers pharmacologists, physiologists, neuroscientists, neurosurgeons, and neurologists an array of powerful tools to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.show more

Review quote

"An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier."-Doody's Health Science Book Review Journalshow more

Table of contents

Part I. Tissue Techniques Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels Sukriti Nag Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry Sukriti Nag Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions Hartwig Wolburg, Stefan Liebner, and Andrea Lippoldt Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy Albert S. Lossinsky and Richard R. Shivers Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy Britta Engelhardt, Peter Vajkoczy, and Melanie Laschinger Part II. Blood-Brain Barrier Permeability Techniques Pathophysiology of Blood-Brain Barrier Breakdown Sukriti Nag Pial Vessel Permeability to Tracers Using Cranial Windows William G. Mayhan Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry Sukriti Nag Ultracytochemical Studies of the Compromised Blood-Brain Barrier Sukriti Nag Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier Eain M. Cornford, Shigeyo Hyman, and Marcia E. Cornford Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers Kamlesh Asotra, Nagendra Ningaraj, and Keith L. Black Part III. Blood-Brain Barrier Transport Techniques A Review of Blood-Brain Barrier Transport Techniques Quentin R. Smith In Situ Brain Perfusion Technique Quentin R. Smith and David D. Allen Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain Barrier Hiroyuki Kusuhara, Tetsuya Terasaki, and Yuichi Sugiyama Transport Studies Using Membrane Vesicles Darryl R. Peterson and Richard A. Hawkins Drug Transport Studies Using Quantitative Microdialysis Haiqing Dai and William F. Elmquist Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids Michelle A. Puchowicz, Kui Xu, and Joseph C. LaManna Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin Laurence Fenart and Romeo Cecchelli Transport Across the Choroid Plexuses In Vivo and In Vitro Nathalie Strazielle and Jane Elizabeth Preston Part IV. In Vitro Techniques An Overview of In Vitro Techniques for Blood-Brain Barrier Studies Andreas Reichel, David J. Begley, and N. Joan Abbott Isolation and Characterization of Human Brain Endothelial Cells Katerina Dorovini-Zis, Rukmini Premeya, and Hanh Huynh Human Brain Microvessel Endothelial Cell and Leukocyte Interactions Jacqueline Shukaliak-Quandt, Donald Wong, and Katerina Dorovini-Zis Development and Characterization of Immortalized Cerebral Endothelial Cell Lines Pierre-Olivier Couraud, John Greenwood, Francoise Roux, and Pete Adamson Isolation and Characterization of Retinal Endothelial Cells David A. Antonetti and Ellen B. Wolpert Isolation and Characterization of Cerebral Microvascular Pericytes Paula Dore-Duffy Part V. Molecular Techniques Molecular Biology of the Blood-Brain Barrier William M. Pardridge Blood-Brain Barrier Genomics Ruben J. Boado Dynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray Analysis Matteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, and Damir Janigro Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells Jamshid Tanha, Arumugam Muruganandam, and Danica Stanimirovic mRNA Detection in Cerebral Vessels by Nonradioactive In Situshow more