Bioconjugate Techniques

Bioconjugate Techniques

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\"Bioconjugate Techniques, 2e\" provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. It is a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab, allowing for fast retrieval of tried and tested techniques for synthesizing bioconjugates.A step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates. It includes more than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates, giving researchers a better understanding of how the commercially available reagents facilitate the crosslinking of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. It includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticles; Silane coupling agents; Dendrimers and dendrons; Chemoselective ligation; Quantum dots; Lanthanide chelates; Cyanine dyes; Discrete PEG compounds; Buckyballs, fullerenes, and carbon nanotubes; Mass tags and isotope tags; and, Bioconjugation in the study of protein interactions.
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Product details

  • Paperback | 1323 pages
  • 191 x 235 x 44mm
  • Academic Press Inc
  • San Diego, United Kingdom
  • English
  • 2nd Revised edition
  • Illustrations
  • 0123705010
  • 9780123705013
  • 1,014,770

Table of contents

Bioconjugate Chemistry
1. Functional Targets
2. The Chemistry of Reactive Groups

Bioconjugate Reagents
3. Zero-Length Cross-Linkers
4. Homobifunctional Cross-linkers
5. Heterobifunctional Cross-linkers
6. Trifunctional Cross-linkers
7. Dendrimers and Dendrons
8. Cleavable Reagent Systems
9. Fluorescent Probes
10. Bifunctional Chelating Agents and Radioimmunoconjugates
11. Biotinylation Reagents
12. Iodination Reagents
13. Silane Coupling Agents
14. Microparticles and Nanoparticles
15. Buckyballs, Fullerenes, and Carbon Nanotubes
16. Mass Tags and Isotope Tags
17. Chemoselective Ligation; Bioorthogonal Reagents
18. Discrete PEG Compounds

Bioconjugate Applications
19. Preparation of Hapten - Carrier Immunogen Conjugates
20. Antibody Modification and Conjugation
21. Immunotoxin Conjugation Techniques
22. Preparation of Liposome Conjugates and Derivatives
23. Avidin - Biotin Systems
24. Preparation of Colloidal-Gold-Labeled Proteins
25. Modification with Synthetic Polymers
26. Enzyme Modification and Conjugation
27. Nucleic Acid and Oligonucleotide Modification and Conjugation
28. Bioconjugation for the Study of Protein Interactions
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